BACKGROUND In strawberry cultivation, continuous cropping (CC) obstacles seriously threaten production. A patented soil amendment (SA) can effectively relieve the CC obstacles to strawberry cultivation, but knowledge of the recovery mechanisms underlying this phenomenon is limited. RESULTS In this study, transcriptomic profiling of strawberry roots in soil with and without the SA was conducted using RNA-Seq technology to reveal gene expression changes in response to SA treatment. In total, 188 differentially expressed genes (DEGs), including 144 upregulated and 44 downregulated DEGs, were identified. SA treatment resulted in genotype-dependent responses, and the response pattern, including an overall increase in the expression of nutrient transport genes and a decrease in the expression of defense response genes, may be a possible mechanism underlying recovery strategies in strawberry roots after the application of the SA to CC soil. We also found that 9 Hsp genes involved in plant defense pathways were all downregulated in the SA-treated roots. CONCLUSIONS This research indicated that strawberry plants reallocated defense resources to development when SA treatment alleviated the stress caused by a CC soil environment. The present study provides an opportunity to reveal the fundamental mechanisms of the tradeoff between growth and defense in strawberry.

中文翻译：

---

转录组分析揭示了在连作土壤中使用土壤改良剂后草莓根的恢复策略。

背景技术在草莓栽培中，连续种植（CC）障碍严重威胁产量。获得专利的土壤改良剂（SA）可以有效缓解CC种植草莓的障碍，但对这种现象背后的恢复机制的了解有限。结果在这项研究中，使用RNA-Seq技术在有或没有SA的土壤中对草莓根进行转录组分析，以揭示响应SA处理的基因表达变化。总共鉴定出188个差异表达基因（DEG），包括144个上调的DEG和44个下调的DEG。SA处理导致基因型依赖性反应和反应模式，包括营养转运基因表达的整体增加和防御反应基因的表达的整体降低，将SA施用到CC土壤上后，可能是草莓根恢复策略的潜在机制。我们还发现参与植物防御途径的9个Hsp基因在SA处理的根中均被下调。结论这项研究表明，当SA处理减轻CC土壤环境造成的胁迫时，草莓植物将防御资源重新分配给了发育。本研究提供了一个机会，揭示了草莓生长与防御之间权衡的基本机制。结论这项研究表明，当SA处理减轻CC土壤环境造成的胁迫时，草莓植物将防御资源重新分配给了发育。本研究提供了一个机会，揭示了草莓生长与防御之间权衡的基本机制。结论这项研究表明，当SA处理减轻CC土壤环境造成的胁迫时，草莓植物将防御资源重新分配给了发育。本研究提供了一个机会，揭示了草莓生长与防御之间权衡的基本机制。