Rifampicin is a common antibiotic used in human and veterinary medicine to treat tuberculosis and other diseases caused by numerous pathogenic bacteria. However, the excessive or improper use of rifampicin usually leads to a series of problems, including bacterial resistance, excessive drug-resistance and water pollution. Thus, it is of great importance to develop selective and sensitive assays for monitoring rifampicin in biological systems. In this study, we designed a fluorescence “turn-off” strategy for the trace detection of rifampicin based on a glutathione-stabilized copper nanoclusters (GSH-Cu NC) sensor. In an aqueous solution, the fluorescence of the GSH-Cu NCs at 632 nm can be quenched effectively and selectively by rifampicin due to the inner-filter effect (IFE) of fluorescence mechanism. Distinctively, this GSH-Cu NC sensor exhibited excellent fluorescence sensing capability for the trace detection of rifampicin with a very low limit of detection (LOD) of 16 pM in a wide linear range from 50 to 10 000 pM. It is not only more sensitive than the other methods previously reported for the detection of rifampicin, but also has an outstanding selectivity and strong anti-interference in complex samples. Furthermore, the as-developed GSH-Cu NCs were also successfully applied to determine rifampicin in different real samples with quantitative spike recoveries ranging from 97% to 105%.

中文翻译：

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基于谷胱甘肽稳定的铜纳米团簇的超灵敏“关断”荧光传感器，用于痕量检测利福平

利福平是人类和兽医学中常用的抗生素，用于治疗由多种病原菌引起的结核病和其他疾病。但是，过量或不当使用利福平通常会导致一系列问题，包括细菌耐药性，过度耐药性和水污染。因此，开发用于监测生物系统中利福平的选择性和灵敏测定非常重要。在这项研究中，我们设计了一种基于谷胱甘肽稳定的铜纳米簇（GSH-Cu NC）传感器的荧光“关闭”策略，用于痕量利福平的检测。在水溶液中，由于荧光机理的内滤效应（IFE），利福平可以有效和选择性地猝灭GSH-Cu NCs在632 nm处的荧光。与众不同 该GSH-Cu NC传感器对氟利平的痕量检测具有出色的荧光感应能力，在50至10000 pM的宽线性范围内，检测限（LOD）极低，仅为16 pM。它不仅比以前报道的其他检测利福平的方法灵敏，而且在复杂样品中具有出色的选择性和强抗干扰性。此外，已开发的GSH-Cu NCs还成功地用于测定不同实际样品中的利福平，其定量加标回收率为97％至105％。它不仅比以前报道的其他检测利福平的方法灵敏，而且在复杂样品中具有出色的选择性和强抗干扰性。此外，已开发的GSH-Cu NCs还成功地用于测定不同实际样品中的利福平，其定量加标回收率为97％至105％。它不仅比以前报道的其他检测利福平的方法灵敏，而且在复杂样品中具有出色的选择性和强抗干扰性。此外，已开发的GSH-Cu NCs还成功地用于测定不同实际样品中的利福平，其定量加标回收率为97％至105％。