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Prognostic value of DNA ploidy and automated assessment of stroma fraction in prostate cancer
International Journal of Cancer ( IF 6.4 ) Pub Date : 2019-12-17 , DOI: 10.1002/ijc.32832
Elin Ersvær 1 , Tarjei S. Hveem 1 , Ljiljana Vlatkovic 1, 2 , Bjørn Brennhovd 3 , Andreas Kleppe 1, 4 , Kari A.R. Tobin 1 , Manohar Pradhan 1 , Karolina Cyll 1 , Håkon Wæhre 1 , David J. Kerr 5 , Håvard E. Danielsen 1, 4, 5
Affiliation  

The combination of DNA ploidy and automatically estimated stroma fraction has been shown to correlate with recurrence and cancer death in colorectal cancer. We aimed to extend this observation and evaluate the prognostic importance of this combined marker in prostate cancer. DNA ploidy status was determined by image cytometry and the stroma fraction was estimated automatically on hematoxylin and eosin stained sections in three tumor samples from each patient to account for tumor heterogeneity. The optimal threshold for low (≤56%) and high (>56%) stroma fraction was identified in a discovery cohort (n = 253). The combined marker was validated in an independent patient cohort (n = 259) with biochemical recurrence as endpoint. The combined marker predicted biochemical recurrence independently in the validation cohort. Multivariable analysis showed that the highest risk of recurrence was observed for patients with samples that had both non‐diploid ploidy status and a high stroma fraction (hazard ratio: 2.51, 95% confidence interval: 1.18–5.34). In conclusion, we suggest the combination of DNA ploidy and automatically estimated stroma fraction as a prognostic marker for the risk stratification of prostate cancer patients. It may also be a potential generic marker as concurrent results have been described in colorectal cancer.

中文翻译:

DNA倍性对前列腺癌的预后价值和间质分数的自动评估

DNA倍性与自动估计的基质分数的组合已显示与大肠癌的复发和癌症死亡相关。我们旨在扩大这一观察范围,并评估这种联合标记物在前列腺癌中的预后重要性。通过图像细胞术确定DNA倍性状态,并在每个患者的三个肿瘤样本中的苏木精和曙红染色切片上自动估算基质分数,以说明肿瘤的异质性。在发现队列中确定了低(≤56%)和高(> 56%)基质分数的最佳阈值(n = 253)。组合标记在一个独立的患者队列中进行了验证(n= 259),以生化复发为终点。组合标记在验证队列中独立预测生化复发。多变量分析显示,既有非二倍体倍性状态又有高基质分数的患者,复发风险最高(危险比:2.51,95%置信区间:1.15-5.34)。总之,我们建议将DNA倍性与自动估计的基质分数结合起来作为前列腺癌患者风险分层的预后标志。它也可能是潜在的通用标记,因为已在结直肠癌中描述了同时发生的结果。
更新日期:2019-12-17
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