当前位置: X-MOL 学术Plant Sci. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Biotechnological, biomedical, and agronomical applications of plant protease inhibitors with high stability: A systematic review
Plant Science ( IF 5.2 ) Pub Date : 2020-03-01 , DOI: 10.1016/j.plantsci.2019.110398
Juliana Cotabarren 1 , Daniela Lufrano 1 , Mónica Graciela Parisi 2 , Walter David Obregón 1
Affiliation  

Protease inhibitors (PIs) are regulatory proteins found in numerous animal tissues and fluids, plants, and microorganisms that reduce and inhibit the exacerbated and uncontrolled activity of the target proteases. Specific PIs are also effective tools for inactivating proteases involved in human diseases like arthritis, pancreatitis, hepatitis, cancer, AIDS, thrombosis, emphysema, hypertension, and muscular dystrophy among others. Plant PIs-small peptides with a high content of cystine residues in disulfide bridges-possess a remarkable resistance to heat treatment and a high stability against shifts in pH, denaturing agents, ionic strength, and proteolysis. In recent years, novel biologic activities have been reported for plant PIs, including antimicrobial, anticoagulant, antioxidant action plus inhibition of tumor-cell growth; thus pointing to possible applications in medicine, agriculture, and biotechnology. In this review, we provide a comparative overview of plant-PIs classifying them in four groups according of their thermal and pH stability (high stability and hyperstable -to temperature and to pHs-, respectively), then emphasizing the relevance of the physicochemical characteristics of these proteins for potential biotechnological and industrial applications. Finally, we analyze the biologic activities of the stable protease inhibitors previously characterized that are the most relevant to potential applications in biomedicine, the food industry, and agriculture.

中文翻译:

高稳定性植物蛋白酶抑制剂的生物技术、生物医学和农艺应用:系统评价

蛋白酶抑制剂 (PI) 是在许多动物组织和体液、植物和微生物中发现的调节蛋白,可降低和抑制目标蛋白酶的加剧和不受控制的活性。特定的 PI 也是灭活涉及人类疾病(如关节炎、胰腺炎、肝炎、癌症、艾滋病、血栓形成、肺气肿、高血压和肌肉萎缩症等)的蛋白酶的有效工具。植物 PIs——二硫键中胱氨酸残基含量高的小肽——对热处理具有显着的抵抗力,对 pH 值、变性剂、离子强度和蛋白水解的变化具有高稳定性。近年来,已经报道了植物 PI 的新生物活性,包括抗菌、抗凝、抗氧化作用以及抑制肿瘤细胞生长;因此指出了在医学、农业和生物技术方面的可能应用。在这篇综述中,我们提供了植物 PI 的比较概述,根据它们的热稳定性和 pH 稳定性(分别对温度和 pH 具有高稳定性和超稳定性)将它们分为四组,然后强调了它们的理化特性的相关性。这些蛋白质具有潜在的生物技术和工业应用。最后,我们分析了先前表征的稳定蛋白酶抑制剂的生物活性,这些抑制剂与生物医学、食品工业和农业的潜在应用最相关。我们提供了植物 PI 的比较概述,根据它们的热稳定性和 pH 稳定性(分别为高稳定性和超稳定性 - 对温度和 pH 值)将它们分为四组,然后强调这些蛋白质的物理化学特征与潜力的相关性生物技术和工业应用。最后,我们分析了先前表征的稳定蛋白酶抑制剂的生物活性,这些抑制剂与生物医学、食品工业和农业的潜在应用最相关。我们提供了植物 PI 的比较概述,根据它们的热稳定性和 pH 稳定性(分别为高稳定性和超稳定性 - 对温度和 pH 值)将它们分为四组,然后强调这些蛋白质的物理化学特征与潜力的相关性生物技术和工业应用。最后,我们分析了先前表征的稳定蛋白酶抑制剂的生物活性,这些抑制剂与生物医学、食品工业和农业的潜在应用最相关。
更新日期:2020-03-01
down
wechat
bug