A novel multilayer modulator chip offering a robust miniaturized interface for multidimensional liquid chromatography has been developed. The thermoplastic microfluidic device comprises five tailor-made functional layers, and the chip is compatible with commercially available switching-valve technology. The modulator chip allows for robust ultrahigh-pressure operation up to 65 MPa. Peak-dispersion characteristics of system peaks were assessed directly at the valve outlet by monitoring fluorescein injection profiles with laser-induced fluorescence detection. Integration of a microporous monolithic mixing entity in the microchannels significantly narrows the resulting peak profile. Proof-of-concept of the applicability of the microfluidic modulator chip is demonstrated in a heart-cut multidimensional strong-cation-exchange-reversed-phase liquid chromatography proteomics analysis workflow coupled to nanoelectrospray mass spectrometry for the target analysis of Glu-1-Fibrinopeptide B spiked in a protein digest mixture of bovine serum albumin.

中文翻译：

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微流控调制器芯片的原型设计及其在针对目标蛋白质组学的纳米切割强阳离子交换反相液相色谱与纳米电喷雾质谱联用中的应用。

已经开发了一种新颖的多层调制器芯片，该芯片为多维液相色谱提供了鲁棒的微型接口。热塑性微流体装置包括五个量身定制的功能层，并且该芯片与市售的开关阀技术兼容。调制器芯片允许高达65 MPa的强大超高压运行。通过用激光诱导的荧光检测监测荧光素注射曲线，直接在阀出口处评估系统峰的峰分散特性。微通道中的微孔整体混合实体的整合显着缩小了所得峰的轮廓。