DNAzymes with nucleic acid-cleaving catalytic activity are increasing in versatility through concerted efforts to discover new sequences with unique functions, and they are generating excitement in the sensing community as cheap, stable, amplifiable detection elements. This review provides a comprehensive list and detailed descriptions of the DNAzymes identified to date, classified by their associated small molecule or ion needed for catalysis; of note, this classification clarifies conserved regions of various DNAzymes that are not obvious in the literature. Furthermore, we detail the breadth of functionality of these DNA sequences as well as the range of reaction conditions under which they are useful. In addition, the utility of the DNAzymes in a variety of sensing and therapeutic applications is presented, detailing both their advantages and disadvantages.

中文翻译：

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核酸裂解催化DNA，用于传感和治疗。

通过共同努力发现具有独特功能的新序列，具有核酸裂解催化活性的DNA酶的多功能性不断提高，它们在传感领域作为廉价，稳定，可扩增的检测元件而引起人们的兴奋。这篇综述提供了迄今为止鉴定出的脱氧核酶的全面列表和详细描述，按催化相关的小分子或离子分类；值得注意的是，这种分类澄清了在文献中不明显的各种DNA酶的保守区域。此外，我们详细介绍了这些DNA序列功能的广度以及它们有用的反应条件范围。此外，还介绍了DNA酶在各种传感和治疗应用中的用途，