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Redox-derivatization reaction-based rapid and sensitive determination of nitrite using resonance Rayleigh scattering method.
Analytical and Bioanalytical Chemistry ( IF 4.3 ) Pub Date : 2020-01-03 , DOI: 10.1007/s00216-019-02333-9
Jincan He 1 , Zihui Zhong 1 , Shiying Tan 1 , Fuhai Wu 1 , Yan Bai 1
Affiliation  

It remains a problem for direct detection of small inorganic nitrite ions using resonance Rayleigh scattering (RRS) method based on the direct dye-binding reaction. In the present study, a redox-derivatization reaction taking only 5 min was introduced prior to nitrite detection. In the redox-derivatization reaction, nitrite ions were reduced by excess iodine ions to generate triiodide ions (I3-), which were further derivatized with a cationic dye (basic violet 1, BV1) to form the ion associates of I3--BV1. Therefore, the RRS signal was significantly enhanced, resulting from the increase of particle size and resonance-enhanced scattering effect. The analytical procedure was performed by just mixing nitrite, oxidant, acid, and dye all-in-one, avoiding the tediousness of a multi-step process or the preparation of nanoparticles. The whole detection process including the redox-derivatization reaction was less than 6 min. The reaction conditions such as concentration of hydrochloric acid, potassium iodide, and BV1, reaction time, and temperature were investigated. Under optimum conditions, the concentration of nitrite was linear with an RRS signal of I3--BV1 ion associates at 320 nm in the range of 0.015-1.2 mg/L. The limit of detection (LOD) was calculated to be 3.0 μg/L. The RRS method was applied to the determination of nitrite in real samples such as pork sausage, milk powder, and water with recovery of 95.2-112%. With advantages of rapidness, high sensitivity, and high selectivity, the method indicates potential applicability for detection of nitrite in complex samples. The method also provides an instructive protocol for detection of analytes that generate no/weak RRS enhancement after the direct dye-binding reaction. Graphical abstract.

中文翻译:

共振瑞利散射法基于氧化还原-衍生化反应快速,灵敏地测定亚硝酸盐。

使用基于直接染料结合反应的共振瑞利散射(RRS)方法直接检测小的无机亚硝酸根离子仍然是一个问题。在本研究中,仅需5分钟即可进行亚硝酸盐检测之前的氧化还原衍生化反应。在氧化还原衍生化反应中,亚硝酸根离子被过量的碘离子还原,生成三碘离子(I3-),然后再用阳离子染料(碱性紫1,BV1)衍生化形成I3--BV1的离子缔合体。因此,由于粒径增加和共振增强的散射效应,RRS信号显着增强。通过仅将亚硝酸盐,氧化剂,酸和染料混合在一起进行分析程序,避免了多步法或制备纳米粒子的繁琐工作。包括氧化还原衍生反应在内的整个检测过程少于6分钟。研究了盐酸,碘化钾和BV1的浓度,反应时间和温度等反应条件。在最佳条件下,亚硝酸盐的浓度与I3-BV1离子缔合的RRS信号在320 nm处的线性关系为0.015-1.2 mg / L。检出限(LOD)计算为3.0μg/ L。RRS方法用于测定猪肉香肠,奶粉和水等真实样品中的亚硝酸盐,回收率为95.2-112%。该方法具有快速,高灵敏度和高选择性的优点,表明该方法可用于检测复杂样品中的亚硝酸盐。该方法还提供了指导性方案,可用于检测直接染料结合反应后不产生/弱RRS增强的分析物。图形概要。
更新日期:2020-01-04
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