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A Class II-Restricted CD8γ13 T-Cell Clone Protects During Chlamydia muridarum Genital Tract Infection.
The Journal of Infectious Diseases ( IF 6.4 ) Pub Date : 2020-05-11 , DOI: 10.1093/infdis/jiz685
Raymond M Johnson 1 , Norma Olivares-Strank 1 , Gang Peng 2
Affiliation  

BACKGROUND The T-cell response to chlamydia genital tract infections in humans and mice is unusual because the majority of antigen-specific CD8 T cells are not class I restricted (referred to here as "unrestricted" or "atypical"). We previously reported that a subset of unrestricted murine chlamydia-specific CD8 T cells had a cytokine polarization pattern that included interferon (IFN)-γ and interleukin (IL)-13. METHODS In this study, we investigated the transcriptome of CD8γ13 T cells, comparing them to Tc1 clones using microarray analysis. That study revealed that CD8γ13 polarization included IL-5 in addition to IFN-γ and IL-13. Adoptive transfer studies were performed with Tc1 clones and a CD8γ13 T-cell clone to determine whether either influenced bacterial clearance or immunopathology during Chlamydia muridarum genital tract infections. RESULTS To our surprise, an adoptively transferred CD8γ13 T-cell clone was remarkably proficient at preventing chlamydia immunopathology, whereas the multifunctional Tc1 clone did not enhance clearance or significantly alter immunopathology. Mapping studies with major histocompatibility complex (MHC) class I- and class II-deficient splenocytes showed our previously published chlamydia-specific CD8 T-cell clones are MHC class II restricted. CONCLUSIONS The MHC class II-restricted CD8 T cells may play an important role in protection from intracellular pathogens that limit class I antigen presentation or diminish CD4 T-cell numbers or impair their function.

中文翻译:

II 类限制性 CD8γ13 T 细胞克隆在鼠衣原体生殖道感染期间提供保护。

背景技术在人和小鼠中对衣原体生殖道感染的 T 细胞反应是不寻常的,因为大多数抗原特异性 CD8 T 细胞不受 I 类限制(此处称为“非限制性”或“非典型”)。我们之前报道过,一部分不受限制的鼠衣原体特异性 CD8 T 细胞具有细胞因子极化模式,包括干扰素 (IFN)-γ 和白细胞介素 (IL)-13。方法 在这项研究中,我们研究了 CD8γ13 T 细胞的转录组,使用微阵列分析将它们与 Tc1 克隆进行比较。该研究表明,除 IFN-γ 和 IL-13 外,CD8γ13 极化还包括 IL-5。使用 Tc1 克隆和 CD8γ13 T 细胞克隆进行过继转移研究,以确定在鼠衣原体生殖道感染期间是否影响细菌清除或免疫病理学。结果 令我们惊讶的是,过继转移的 CD8γ13 T 细胞克隆在预防衣原体免疫病理学方面非常擅长,而多功能 Tc1 克隆并未增强清除或显着改变免疫病理学。主要组织相容性复合体 (MHC) I 类和 II 类缺陷脾细胞的映射研究表明,我们之前发表的衣原体特异性 CD8 T 细胞克隆受 MHC II 类限制。
更新日期:2020-01-04
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