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The RALF1-FERONIA Complex Phosphorylates eIF4E1 to Promote Protein Synthesis and Polar Root Hair Growth.
Molecular Plant ( IF 27.5 ) Pub Date : 2020-01-03 , DOI: 10.1016/j.molp.2019.12.014
Sirui Zhu 1 , José Manuel Estévez 2 , Hongdong Liao 1 , Yonghua Zhu 1 , Tao Yang 3 , Chiyu Li 1 , Yichuan Wang 4 , Lan Li 1 , Xuanming Liu 1 , Javier Martinez Pacheco 5 , Hongwei Guo 4 , Feng Yu 6
Affiliation  

The molecular links between extracellular signals and the regulation of localized protein synthesis in plant cells are poorly understood. Here, we show that in Arabidopsis thaliana, the extracellular peptide RALF1 and its receptor, the FERONIA receptor kinase, promote root hair (RH) tip growth by modulating protein synthesis. We found that RALF1 promotes FERONIA-mediated phosphorylation of eIF4E1, a eukaryotic translation initiation factor that plays a crucial role in the control of mRNA translation rate. Phosphorylated eIF4E1 increases mRNA affinity and modulates mRNA translation and, thus, protein synthesis. The mRNAs targeted by the RALF1–FERONIA–eIF4E1 module include ROP2 and RSL4, which are important regulators of RH cell polarity and growth. RALF1 and FERONIA are expressed in a polar manner in RHs, which facilitate eIF4E1 polar localization and thus may control local ROP2 translation. Moreover, we demonstrated that high-level accumulation of RSL4 exerts negative-feedback regulation of RALF1 expression by directly binding the RALF1 gene promoter, determining the final RH size. Our study reveals that the link between RALF1–FERONIA signaling and protein synthesis constitutes a novel component regulating cell expansion in these polar growing cells.



中文翻译:

RALF1-FERONIA复合物使eIF4E1磷酸化,从而促进蛋白质合成和极根毛发生长。

人们对细胞外信号与植物细胞中局部蛋白质合成调控之间的分子联系了解甚少。在这里,我们表明在拟南芥中,细胞外肽RALF1及其受体FERONIA受体激酶通过调节蛋白质合成来促进根毛(RH)尖端生长。我们发现RALF1促进FEIFIA介导的eIF4E1的磷酸化,eIF4E1是一种真核翻译起始因子,在控制mRNA的翻译速率中起关键作用。磷酸化的eIF4E1增加了mRNA的亲和力并调节了mRNA的翻译,从而调节了蛋白质的合成。RALF1-FERONIA-eIF4E1模块靶向的mRNA包括ROP2和RSL4,它们是RH细胞极性和生长的重要调节剂。RALF1和FERONIA在RH中以极性方式表达,这有助于eIF4E1极性定位,因此可以控制局部ROP2翻译。此外,我们证明了RSL4的高水平积累通过直接结合RALF1基因启动子,确定最终的RH大小而对RALF1表达产生负反馈调节。我们的研究表明,RALF1-FERONIA信号传导与蛋白质合成之间的联系构成了调节这些极性生长细胞中细胞扩增的新成分。

更新日期:2020-01-03
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