Owing to superior safety, the RNA-only delivery synthetic circuit is more suitable for cell-based medicine. Modules which possess matching inputs and outputs could be strung by daisy-chaining to compose RNA-only delivery synthetic gene circuits. In this study, we engineered well-characterized biological parts to construct composable modules, each of which could receive signals from the upstream module and transmit the processed signal to the next module using standard interfaces. Capsid-cNOT7, through which logic gates could be changed by merely changing the type of it, was used as the core element for logical process. Daisy chain topology was used to build RNA-only delivery mammalian synthetic circuits which possess validated functions such as fan out, protein sensing, drug sensing, light sensing, 2-input logic gate, 3-input logic gate, and volatile memory, providing a new method to simplify the design of RNA-only delivery synthetic circuits.

中文翻译：

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基于菊花链拓扑的仅用于RNA传递的哺乳动物合成电路。

由于具有优越的安全性，仅RNA的递送合成电路更适合用于基于细胞的药物。具有匹配输入和输出的模块可以通过菊花链串接起来，组成仅RNA的传递合成基因电路。在这项研究中，我们设计了功能完备的生物部件，以构建可组合的模块，每个模块都可以从上游模块接收信号，并使用标准接口将处理后的信号传输到下一个模块。通过仅更改类型即可更改逻辑门的Capsid-cNOT7用作逻辑过程的核心元素。菊花链拓扑结构用于构建仅RNA的哺乳动物传递合成回路，该回路具有经过验证的功能，例如扇出，蛋白质传感，药物传感，光传感，2输入逻辑门，3输入逻辑门，