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Effects of prednisolone on behavior and hypothalamic-pituitary-interrenal axis activity in zebrafish.
Environmental Toxicology and Pharmacology ( IF 4.3 ) Pub Date : 2020-01-03 , DOI: 10.1016/j.etap.2020.103325
Ning Xin 1 , Yu Jiang 2 , Sha Liu 1 , Yanlong Zhou 1 , Yanbo Cheng 1
Affiliation  

Prednisolone is a synthetic glucocorticoid used clinically for treating allergies, inflammation, and autoimmune diseases. Long-term prednisolone use has been shown to have negative effects on physiology and mood. We aimed to study the pharmacology and toxicology of glucocorticoid-like drugs by investigating behavioral and hypothalamic–pituitary–interrenal (HPI) axis effects in a zebrafish model. Zebrafish embryos 24 h post fertilization were exposed to 25 μM prednisolone. Their behavior was investigated 5 days post fertilization (dpf), and their HPI axis-related activity and related neurotransmitter levels were investigated 3, 4, 5, and 6 dpf. The behavior results showed that exposure to prednisolone resulted in decreased autonomic activity and low sensitivity to light. qRT-PCR and ELISA results showed decreased activity of the HPI axis and increased secretion of dopamine and serotonin after exposure to prednisolone. This study provides us with new insights into understanding the effects of glucocorticoids on the HPI axis.



中文翻译:

泼尼松龙对斑马鱼行为和下丘脑-垂体-肾间轴活动的影响。

泼尼松龙是一种合成的糖皮质激素,临床上用于治疗过敏,炎症和自身免疫性疾病。长期使用泼尼松龙已被证明对生理和情绪有负面影响。我们旨在通过研究斑马鱼模型中的行为和下丘脑-垂体-肾间(HPI)轴效应来研究糖皮质激素样药物的药理学和毒理学。受精后24小时将斑马鱼胚胎暴露于25μM泼尼松龙中。在受精(dpf)5天后调查他们的行为,并调查3、4、5和6 dpf他们与HPI轴相关的活性和相关的神经递质水平。行为结果表明,强的松龙的暴露导致自主神经活动的减少和对光的低敏感性。qRT-PCR和ELISA结果显示,泼尼松龙暴露后HPI轴活性降低,多巴胺和5-羟色胺分泌增加。这项研究为我们提供了新的见解,以了解糖皮质激素对HPI轴的影响。

更新日期:2020-01-03
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