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Assessing the stability of historical and desiccated snake venoms from a medically important Ecuadorian collection.
Comparative Biochemistry and Physiology C: Toxicology & Pharmacology ( IF 3.9 ) Pub Date : 2020-01-03 , DOI: 10.1016/j.cbpc.2020.108702
José R Almeida 1 , Bruno Mendes 2 , Ricardo S P Patiño 1 , José Pico 3 , Johanna Laines 3 , María Terán 3 , Noroska G S Mogollón 1 , Fausto Zaruma-Torres 4 , Cleópatra A da S Caldeira 5 , Saulo L da Silva 4
Affiliation  

Bothrops asper and Bothrops atrox are important venomous snakes from Ecuador responsible for the most of ophidic accidents, which in the past were treated with a national polyvant antivenom. For years, the venom pools were collected and stored at room temperature in a laboratory. Taking into account the controversial ability of desiccated samples to retain their biological effects and enzymatic activities, we investigated the biochemical and toxicological properties of venoms after years of storage. The proteomic profiles of historical venoms analyzed by high-performance liquid chromatography and electrophoresis are very similar. The fresh batches of venom were more lethal than those stored for years, just as the initial and current LD50 values of these samples changed. Significant differences were showed in the myotoxic and hemorrhagic activity of some venom pools, while no significant statistical differences were found for the edema activity. The enzymatic assays revealed a variation in proteolytic activity on azocasein and phospholipase A2 activity, and low differences were reported for thrombin-like serine protease activity. The maintenance of the proteomic profile and certain toxicological activities convert this venom library in a valuable source for research purposes. Nonetheless, the significative reduction of toxicological activities, such as hemorrhagic activity not feasible using these samples for the antivenom production.

中文翻译:

从医学上重要的厄瓜多尔收藏中评估历史悠久的和干燥的蛇毒的稳定性。

Bothrops asper和Bothrops atrox是厄瓜多尔重要的有毒蛇,它们造成了大部分的哲学事故,这些事故在过去曾被国家多抗蚁药处理。多年来,毒液池被收集并在室温下在实验室中存储。考虑到干燥样品保留其生物学效应和酶促活性的争议能力,我们调查了多年储存后毒液的生化和毒理学性质。通过高效液相色谱和电泳分析的历史毒液的蛋白质组学特征非常相似。新鲜批次的毒液比储存多年的毒液更具致死性,就像这些样品的初始和当前LD50值改变一样。在某些毒液库的肌毒性和出血活性方面显示出显着差异,而在浮肿活性方面未发现显着的统计学差异。酶促测定揭示了对偶氮酪蛋白和磷脂酶A2活性的蛋白水解活性的变化,并且报道了凝血酶样丝氨酸蛋白酶活性的低差异。蛋白质组学特征的维持和某些毒理学活动可将这种毒液文库转化为有价值的研究用途。然而,使用这些样品生产抗蛇毒血清不能明显降低毒理学活性,例如出血活性。酶促测定揭示了对偶氮酪蛋白和磷脂酶A2活性的蛋白水解活性的变化,并且报道了凝血酶样丝氨酸蛋白酶活性的低差异。蛋白质组学特征的维持和某些毒理学活动可将这种毒液文库转化为有价值的研究用途。然而,使用这些样品生产抗蛇毒血清不能明显降低毒理学活性,例如出血活性。酶促测定揭示了对偶氮酪蛋白和磷脂酶A2活性的蛋白水解活性的变化,并且报道了凝血酶样丝氨酸蛋白酶活性的低差异。蛋白质组学特征的维持和某些毒理学活动可将这种毒液文库转化为有价值的研究用途。然而,使用这些样品生产抗蛇毒血清不能明显降低毒理学活性,例如出血活性。
更新日期:2020-01-04
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