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The temporary modulation of tyramine on immune responses, carbohydrate metabolism, and catecholamines in Macrobrachium rosenbergii.
Fish & Shellfish Immunology ( IF 4.7 ) Pub Date : 2020-01-03 , DOI: 10.1016/j.fsi.2019.12.091
Chin-Chyuan Chang , Hsin-Wei Kuo , Chang-Chi Liu , Winton Cheng

Tyramine (TA), a biogenic monoamine, plays various important physiological roles including immunological regulation in invertebrates. In this study, the effects of TA on the regulation of immune resistance, carbohydrate metabolism and biogenic monoamine, as well as its signaling pathway in Macrobrachium rosenbergii were determined. Results showed that total haemocyte count, hyaline cells, semigranular cells, and phenoloxidase activity per 50 μL of haemolymph and per granulocyte (the sum of semigranular and granular cells) at 0.5 h as well as phagocytic activity and clearance efficiency to Lactococcus garvieae at 1 h of prawn injected with TA at 1 nmol prawn-1 significantly increased, but the significantly decreased plasma lysozyme activity, phagocytic activity, clearance efficiency, and haemolymph glucose and dopamine were observed in prawn injected with TA at 10 nmol prawn-1 for 0.5 h. Respiratory bursts and haemolymph lactate in two TA-injection treatments at 0.5 h and 0.5-1 h, respectively, were significantly higher than those of the saline control, and in addition, TA depressed dopamine release in a dose-dependent manner after 0.5 h of TA injection. All the examined parameters returned to control levels after prawn injected with TA for 2 h. The inhibited effect of TA (at 10 nmol prawn-1 injection) on the phagocytic activity and clearance efficiency to pathogens was blocked by prazosin (an α1 adrenoceptors antagonist). For prawn received TA for 1 h then challenged with Lactococcus garvieae at 2 × 105 colony-forming units prawn-1, the survival ratio of TA 1 nmol prawn-1-injected prawn significantly increased by 20%, compared to the saline-challenged control or TA 10 nmol prawn-1-injected prawn after 144 h of challenge. These results suggested that the level of dopamine release suppression regulated by TA resulted in the immunoenhancing or immunosuppressive effects in prawn, and the signaling pathways of TA in mediating immune function were through octopamine (OA)/TA receptors.

中文翻译:

罗氏沼虾中酪胺对免疫反应,碳水化合物代谢和儿茶酚胺的暂时调节。

酪胺(TA)是一种生物单胺,在无脊椎动物中起着各种重要的生理作用,包括免疫调节。在这项研究中,确定了TA对罗氏沼虾免疫抵抗,碳水化合物代谢和生物单胺的调节及其信号传导途径的影响。结果显示,在0.5 h时,每50μL血淋巴和每粒粒细胞的总血细胞计数,透明质细胞,半颗粒细胞和酚氧化酶活性(半颗粒和颗粒细胞的总和),在1 h时对吞噬乳杆菌的吞噬活性和清除效率在1 nmol prawn-1处注射TA的对虾数量显着增加,但血浆溶菌酶活性,吞噬活性,清除效率显着降低,在10 nmol prawn-1处注射TA 0.5h的虾体内观察到血和血中的葡萄糖和多巴胺含量。两种TA注射治疗分别在0.5 h和0.5-1 h的呼吸爆发和乳酸血淋巴显着高于生理盐水对照组,此外,TA在0.5 h注射后以剂量依赖性方式抑制多巴胺释放。 TA注射。对虾注射TA 2 h后,所有检查的参数均恢复到对照水平。TA(10 nmol prawn-1注射)对吞噬活性和对病原体清除效率的抑制作用被prazosin(α1肾上腺素受体拮抗剂)阻断。对于接受TA 1小时的虾,然后以2×105菌落形成单位prawn-1攻击加乳杆菌,将TA 1 nmol prawn-1注入的虾的存活率显着提高了20%,攻击后144小时,与盐水激发的对照或TA 10 nmol对虾1注射对虾相比。这些结果表明,TA调节的多巴胺释放抑制水平导致虾的免疫增强或免疫抑制作用,而TA介导免疫功能的信号传导途径是通过章鱼胺(OA)/ TA受体。
更新日期:2020-01-04
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