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Ghrelin modulates the immune response and increases resistance to Aeromonas hydrophila infection in hybrid tilapia.
Fish & Shellfish Immunology ( IF 4.7 ) Pub Date : 2020-01-03 , DOI: 10.1016/j.fsi.2020.01.006
Zhuojun Han 1 , Yi Zhou 2 , Xiaojin Zhang 3 , Jinpeng Yan 4 , Jun Xiao 2 , Yongju Luo 3 , Huifang Zheng 5 , Huan Zhong 2
Affiliation  

Ghrelin is a peptide hormone secreted by gastrointestinal tract which regulates multiple physiological processes such as appetite, metabolism, growth and gonad development in fish. In the present study, the effects of ghrelin on hybrid tilapia infected with Aeromonas hydrophila are elucidated. Juvenile hybrid tilapia fish (20.0 ± 5.0 g) were intraperitoneally injected with 0, 0.1, 1.0, or 10.0 ng/g ghrelin/body weight synthetic ghrelin alone or in combination with A. hydrophila (0.5 × 106 CFU). At 10 days post treatment, the survival rate in the group that received 1.0 ng/g ghrelin/body weight ghrelin in combination with A. hydrophila was higher (66.66%) than that of the Ah group (13.33%) that received A. hydrophila alone. In tilapia that received ghrelin injections, reactive oxygen species (ROS) levels tended to increase at 5 h, while injection of 10.0 ng/g ghrelin/body weight ghrelin resulted in a significant decrease in ROS levels at 10 h. No changes in serum immune or antioxidant-related indicators were observed in fish injected with A. hydrophila compared to controls. However, ghrelin injection decreased Albumin (ALB), glutathione peroxidase (GSH-Px), lysozyme (LZM) and superoxide dismutase (SOD). Histological analysis showed that ghrelin injection alleviated the pathological changes in liver and spleen caused by A. hydrophila infection. Overall, the expression of HSP70, IL-1β, and TGF-β in the liver tended to upregulate compared to the control. In the kidney, HSP70, IL-1β and TGF-β levels were increased, and TNF-α expression levels were decreased compared to the control. The HSP70 level in the spleen was decreased, and IL-1β, TGF-β, and TNF-α were expressed at significantly higher levels in the spleen in the tilapia that received ghrelin injections. Taken together, our results indicate that injection with 1.0 ng/g ghrelin/body weight ghrelin may effectively protect juvenile hybrid tilapia against A. hydrophila infection by improving hematological indicators, maintaining normal histology and regulating cytokine gene expression.

中文翻译:

Ghrelin调节杂交罗非鱼中的免疫反应并增加对亲水气单胞菌感染的抵抗力。

Ghrelin是胃肠道分泌的一种肽激素,可调节鱼的食欲,代谢,生长和性腺发育等多种生理过程。在本研究中,阐明了ghrelin对感染嗜水气单胞菌的杂交罗非鱼的作用。分别向幼体杂交罗非鱼(20.0±5.0 g)腹膜内注射0、0.1、1.0或10.0 ng / g ghrelin /体重合成的ghrelin或与亲水性曲霉(0.5×106 CFU)组合。在治疗后第10天,接受1.0 ng / g生长激素释放肽/体重生长激素释放肽与亲水性链球菌联合治疗的组的生存率(66.66%)高于接受亲水性链球菌的Ah组的存活率(13.33%)单独。在接受生长素释放肽注射的罗非鱼中,活性氧(ROS)水平在5小时时趋于增加,而注射10.0 ng / g ghrelin /体重ghrelin则可在10 h时显着降低ROS水平。与对照组相比,在注射亲水性链球菌的鱼中未观察到血清免疫或抗氧化剂相关指标的变化。然而,ghrelin注射会降低白蛋白(ALB),谷胱甘肽过氧化物酶(GSH-Px),溶菌酶(LZM)和超氧化物歧化酶(SOD)。组织学分析表明,生长素释放肽注射液减轻了由亲水性链球菌感染引起的肝脏和脾脏的病理变化。总体而言,与对照组相比,肝脏中HSP70,IL-1β和TGF-β的表达趋于上调。与对照组相比,肾脏中的HSP70,IL-1β和TGF-β水平升高,而TNF-α表达水平降低。脾脏HSP70水平降低,IL-1β,TGF-β,接受生长素释放肽注射的罗非鱼的脾脏中TNF-α和TNF-α的表达明显较高。综上所述,我们的结果表明,注射1.0 ng / g ghrelin /体重ghrelin可以改善血液学指标,维持正常组织学和调节细胞因子基因表达,从而有效地保护少年罗非鱼免受亲水性罗非鱼感染。
更新日期:2020-01-04
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