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Effects of the proportion of two different cross-linkers on the material and biological properties of enzymatically degradable PEG hydrogels
Polymer Degradation and Stability ( IF 5.9 ) Pub Date : 2020-01-03 , DOI: 10.1016/j.polymdegradstab.2019.109067
Siju Liu , Hong Cao , Renqi Guo , Heling Li , Cuifen Lu , Guichun Yang , Junqi Nie , Feiyi Wang , Nianguo Dong , Jiawei Shi , Feng Shi

A series of enzymatically degradable PEG hydrogels with tunable degradability were prepared by changing the proportions of two cross-linkers, enzymatically degradable cross-linker matrix metalloproteinase (MMP) degradable peptide and non-degradable cross-linker PEG-dithiol, and the effects of the proportion of two cross-linkers on the other properties of PEG hydrogels were also investigated. As the content of the degradable cross-linker increased, their mechanical properties decreased, but their swelling rate gradually increased. LIVE/DEAD cell staining and CCK-8 assay indicated that PEG hydrogels have high biocompatibility and low cytotoxicity, and as the content of the degradable cross-linker increased, the proliferation of cells is more favorable. Furthermore, adipogenic differentiation and osteogenic differentiation assays showed that BMSCs 3D-capsulated in PEG hydrogels can proliferate and then differentiate into osteoblasts or adipocytes, and as the content of degradable cross-linker increased, calcium nodules and the number of fat cells increased. These results demonstrate that the proportions of two cross-linkers have an effect on the physical, chemical and biological properties of PEG hydrogels, and these PEG hydrogels could be used as tissue engineering scaffold materials.



中文翻译:

两种不同交联剂的比例对可酶降解PEG水凝胶的材料和生物学特性的影响

通过改变两种交联剂,可酶降解的交联剂基质金属蛋白酶(MMP)可降解肽和不可降解的交联剂PEG-二硫醇的比例,制备了一系列具有可调节降解性的可酶降解的PEG水凝胶。还研究了两种交联剂的比例对PEG水凝胶其他性能的影响。随着可降解交联剂含量的增加,其机械性能下降,但其溶胀速率逐渐提高。LIVE / DEAD细胞染色和CCK-8分析表明PEG水凝胶具有高生物相容性和低细胞毒性,并且随着可降解交联剂含量的增加,细胞增殖更加有利。此外,成脂分化和成骨分化试验表明,3D封装在PEG水凝胶中的BMSC可以增殖然后分化为成骨细胞或脂肪细胞,并且随着可降解交联剂含量的增加,钙结节和脂肪细胞的数量增加。这些结果证明两种交联剂的比例对PEG水凝胶的物理,化学和生物学性质有影响,并且这些PEG水凝胶可用作组织工程支架材料。

更新日期:2020-01-04
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