A new method of screening was developed to generate 770 organic and water-soluble fractions from extracts of nine species of marine sponges, from the growth media of 18 species of marine-derived fungi, and from the growth media of 13 species of endophytic fungi. The screening results indicated that water-soluble fractions displayed significant bioactivity in cytotoxic, antibiotic, anti-Leishmania, anti-Trypanosoma cruzi, and inhibition of proteasome assays. Purification of water-soluble fractions from the growth medium of Penicillium solitum IS1-A provided the new glutamic acid derivatives solitumine A (1), solitumine B (2), and solitumidines A-D (3-6). The structures of compounds 1-6 have been established by analysis of spectroscopic data, chemical derivatizations, and vibrational circular dichroism calculations. Although no biological activity could be observed for compounds 1-6, the new structures reported for 1-6 indicate that the investigation of water-soluble natural products represents a relevant strategy in finding new secondary metabolites.

中文翻译：

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南极海洋国王乔治岛的Solicumium solitum IS1-A在文化中产生的水溶性谷氨酸衍生物。

开发了一种新的筛选方法，可从9种海洋海绵的提取物，18种海洋真菌的生长培养基以及13种内生真菌的生长培养基中生成770种有机和水溶性级分。筛选结果表明，水溶性级分在细胞毒性，抗生素，抗利什曼原虫症，抗克鲁氏锥虫和蛋白酶体测定的抑制作用中显示出显着的生物活性。从青霉IS1-A的生长培养基中纯化水溶性级分提供了新的谷氨酸衍生物solitumine A（1），solitumine B（2）和solumumidines AD（3-6）。化合物1-6的结构已通过光谱数据分析，化学衍生化和振动圆二色性计算确定。