The 3D organization of chromatin plays an important role in genome stability and many other pivotal biological programs. Therefore, the establishment of imaging methods, which enable us to study the dynamics of chromatin in living cells, is necessary. Although primary live cell imaging methods were a breakthrough, there is a need to develop more specific labeling techniques. With the discovery of programmable DNA binding proteins, such zinc finger proteins (ZFP), transcription activator-like effectors (TALE), and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9), a major leap forward was made. Here, we review the applications and potential of fluorescent repressor-operator systems, programmable DNA binding proteins with an emphasis on CRISPR-based chromatin imaging in living and fixed cells, and their potential application in plant science.

中文翻译：

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基于CRISPR / Cas9的方法在活的和固定的植物细胞中追踪确定的基因组序列的应用和前景。

染色质的3D组织在基因组稳定性和许多其他关键生物学程序中起着重要作用。因此，有必要建立使我们能够研究活细胞中染色质动力学的成像方法。尽管主要的活细胞成像方法是一项突破，但仍需要开发更具体的标记技术。随着可编程DNA结合蛋白的发现，例如锌指蛋白（ZFP），转录激活因子样效应物（TALE）和成簇规则间隔的短回文重复序列（CRISPR）/ CRISPR相关蛋白9（Cas9），这是一个重大的飞跃被制造。在这里，我们回顾了荧光阻遏物-操纵子系统，可编程DNA结合蛋白的应用和潜力，重点是活体和固定细胞中基于CRISPR的染色质成像，