Abstract

Brachychiton populneus is one of the unexploited Tunisian plants, traditionally eaten as food and used for medicinal purposes. The present study aimed to investigate the phytochemical components of the seeds, leaves and flowers from B. populneus using three different solvents and to explore their antioxidant, anti-inflammatory and neuroprotective effects. Further, this study was focused on the identification of phenolic compounds from the most active extract. In vitro, all extracts showed strong antioxidant property by DPPH, ferrous ion chelating and lipid peroxidation-inhibiting assays, noticeable anti-inflammatory activity by protein denaturation and membrane stabilization methods and important neuroprotective effects by acetylcholinesterase inhibitory test. In vivo, B. populneus (50, 100 and 200 mg/kg, i.p.) showed significant dose–response anti-inflammatory effects against carrageenan-induced paw edema. With respect to the phenolic profile, the leaf methanol extract presented eight phenolic acids, one flavone and four flavonoids, with salvianolic acid B (820.3 mg/kg), caffeic acid (224.03 mg/kg), syringic acid (100.2 mg/kg) and trans-ferulic acid (60.02 mg/kg) as the major compounds. The results of the current study suggested that B. populneus could be a precious source of health-benefitting biomolecules and may be developed as new antioxidant, anti-inflammatory and AChE inhibitors.

Graphic abstract

中文翻译：

---

短枝曲霉是一种具有治疗作用的生物活性成分的新型来源：抗氧化剂，酶抑制剂，抗炎特性和LC-ESI-MS特性。

摘要

Brachychiton populneus是未开发的突尼斯植物之一，传统上被当作食物食用并用于医药目的。本研究旨在使用三种不同的溶剂调查民用芽孢杆菌种子，叶片和花朵的植物化学成分，并探讨其抗氧化，抗炎和神经保护作用。此外，该研究集中于从活性最高的提取物中鉴定酚类化合物。在体外，所有提取物通过DPPH，亚铁离子螯合和脂质过氧化抑制分析均显示出强大的抗氧化性能，通过蛋白质变性和膜稳定化方法显示出显着的抗炎活性，并通过乙酰胆碱酯酶抑制试验显示出重要的神经保护作用。在体内，流行芽孢杆菌（50、100和200 mg / kg，腹腔注射）对角叉菜胶诱发的爪水肿显示出显着的剂量反应抗炎作用。关于酚类特征，叶甲醇提取物含有八种酚酸，一种黄酮和四种类黄酮，其中丹酚酸B（820.3 mg / kg），咖啡酸（224.03 mg / kg），丁香酸（100.2 mg / kg）主要成分是反式阿魏酸（60.02 mg / kg）。目前的研究结果表明，流行芽孢杆菌可能是有益健康的生物分子的宝贵来源，并可能被开发为新的抗氧化剂，抗炎剂和AChE抑制剂。

图形摘要