To assess risk, the European Food Safety Authority requires that the amino-acid sequences of newly expressed proteins in genetically engineered (GE) crops should be searched for partial matches with 9-mer restricted epitopes known to cause celiac disease. None of the 26 known celiac-causing 9-mer epitopes contain an in-silico predicted trypsin cleavage site. The probability of this occurring by chance alone is 0.000056. Based on the absence of in-silico predicted trypsin cleavage sites within 9-mer epitopes known to cause celiac disease, it can be concluded with very high confidence that true celiac-causing epitopes are highly unlikely to contain in-silico predicted trypsin cleavage sites and that this criterion can reliably be used to exclude the risk that imperfect 9-mer peptide matches within newly expressed proteins from GE crops cause celiac disease.

中文翻译：

---

胰蛋白酶切割位点在引起腹腔的限制性表位中极不可能发生。

为了评估风险，欧洲食品安全局要求对基因工程（GE）作物中新表达的蛋白质的氨基酸序列进行搜索，以寻找与已知会导致乳糜泻的9-mer限制性表位的部分匹配。26个已知的引起乳糜泻的9-mer表位中没有一个在计算机上预测的胰蛋白酶切割位点。仅偶然地发生这种情况的概率为0.000056。基于已知会导致乳糜泻的9-mer表位中没有计算机模拟的胰蛋白酶切割位点，