Cryo-EM technology has developed to the point of high-throughput structure determination of biological macromolecules embedded in vitreous ice. Nonetheless, challenging targets need extensive sample screening, often of many cryo-EM sample grids prepared under various conditions. We have designed and made tools for manipulating sample grids in storage cases. These tools are made of a plastic fiber using a wide-use 3D printer, a fused deposition modeling type, and polished under acetone gas. A grid case stacker organizes many frozen-hydrated cryo-EM grids and the stackers can be piled up inside a standard 50 mL centrifuge tube. We have also introduced tools that facilitate handling of grid cases under liquid nitrogen and a stocker of the grid retainers contained in a CRYO ARM electron microscope. Blueprints of the tools named CryoGridTools are available from a GitHub site.

中文翻译：

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使用3D打印工具整理cryo-EM样品网格。

Cryo-EM技术已经发展到高通量结构测定玻璃冰中嵌入的生物大分子的地步。然而，具有挑战性的目标需要广泛的样品筛选，通常是在各种条件下制备的许多冷冻-EM样品栅格。我们设计和制造了用于在存储箱中处理样品网格的工具。这些工具由塑料纤维制成，使用了广泛使用的3D打印机，熔融沉积建模类型，并在丙酮气体下进行了抛光。格栅箱堆垛机可组织许多冷冻水合的cryo-EM格栅，并且可以将其堆积在标准的50 mL离心管内。我们还推出了有助于在液氮下处理栅格盒的工具以及CRYO ARM电子显微镜中包含的栅格保持器的储料器。