The Colorado potato beetle (Leptinotarsa decemlineata [Say]) is an insect pest that can significantly harm potato plants worldwide. Control of this insect relies heavily on chemical insecticides such as chlorantraniliprole. Nevertheless, the complete molecular signature associated with response to this compound is lacking in L. decemlineata. In this study, amplification and quantification by qRT‐PCR (quantitative reverse transcription‐polymerase chain reaction) of targets relevant to chlorantraniliprole were undertaken in insects exposed to this chemical. This approach showed modulation of numerous cytochrome P450s, such as CYP350D1 and CYP4Q3, as well as upregulation of microRNAs (miRNAs), including miR‐1‐3p and miR‐305‐5p, in chlorantraniliprole‐exposed insects. Functional assessment of transcript targets predicted to be regulated by these miRNAs was performed and revealed their likely impact on transcriptional regulation. RNAi‐based targeting of CYP350D1 notably provided preliminary evidence of its underlying implication for chlorantraniliprole response in L. decemlineata. Overall, this study strengthens the current knowledge of the molecular changes linked to chlorantraniliprole response in L. decemlineata and provides novel targets with potential relevance to chlorantraniliprole susceptibility in this insect pest of global relevance.

中文翻译：

---

与科罗拉多马铃薯甲虫Leptinotarsa decemlineata中的氯antlaniliprole反应潜在相关的转录本的差异表达。

科罗拉多马铃薯甲虫（Leptinotarsa decemlineata [Say]）是一种害虫，可以严重危害全世界的马铃薯植物。对这种昆虫的控制在很大程度上依赖于化学杀虫剂，例如氯虫腈。然而，在L. decemlineata中缺乏与此化合物相关的完整分子标记。。在这项研究中，通过qRT-PCR（定量逆转录-聚合酶链反应）对与扑虫腈相关的靶标进行了扩增和定量分析。这种方法显示了在暴露于氯吡虫啉的昆虫中，许多细胞色素P450的调节，例如CYP350D1和CYP4Q3，以及microRNA（miRNA）的上调，包括miR-1-3p和miR-305-5p。对预计受这些miRNA调控的转录目标进行功能评估，发现它们可能对转录调控产生影响。CYP350D1的基于RNAi的靶向尤其提供了初步证据，证明其对decemlineata中的chlorantraniliprole反应具有潜在的影响。总的说来，这项研究加强了与L. decemlineata中氯虫腈的响应有关的分子变化的最新知识，并提供了与这种全球相关害虫中氯虫腈敏感性相关的新靶标。