Microbial genomes encode numerous biosynthetic gene clusters (BGCs) that may produce natural products with diverse applications in medicine, agriculture, the environment, and materials science. With the advent of genome sequencing and bioinformatics, heterologous expression of BGCs is of increasing interest in bioactive natural product (NP) discovery. However, this approach has had limited success because expression of BGCs relies heavily on the physiology of just a few commonly available host chassis. Expanding and diversifying the chassis portfolio for heterologous BGC expression may greatly increase the chances for successful NP production. In this review, we first discuss genetic and genome engineering technologies used to clone, modify, and transform BGCs into multiple strains and to engineer chassis strains. We then highlight studies that employed the multi-chassis approach successfully to optimize NP production, discover previously uncharacterized NPs, and better understand BGC function.

中文翻译：

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用于微生物天然产物异源生产的多机箱工程。

微生物基因组编码许多生物合成基因簇（BGC），这些簇可能产生天然产物，在医学，农业，环境和材料科学中具有多种应用。随着基因组测序和生物信息学的出现，BGC的异源表达对生物活性天然产物（NP）的发现越来越感兴趣。但是，这种方法取得的成功有限，因为BGC的表达严重依赖于少数几个常用主机架的生理特性。扩展和多样化用于异源BGC表达的底盘产品组合可能会大大增加成功生产NP的机会。在这篇综述中，我们首先讨论了遗传和基因组工程技术，该技术可用于将BGC克隆，修饰和转化为多种菌株，并设计底盘菌株。