Developmental origins of health and disease (DOHaD) is a field of biological science dedicated to investigating how different interventions during development affect an individual's life. Diet is an essential way to interact with the environment, and during pregnancy affects not only the mother but also can impact the next generations. One of these interventions is caloric restriction (CR), which has shown positive redox modulation in rats' offspring when malnutrition is responsibly controlled. Considering that mitochondrial metabolism is determinant for redox status, we investigated parameters related to mitochondrial functionality and reactive species levels in offspring's brain from rats delivered to pregnant caloric restricted dams. Therefore, pregnant rats were divided between control (ad libitum food) and CR (20% food restriction plus micronutrients supplementation) groups, and offspring's brain was analyzed on post‐natal days (PND) 0, 7, 21, and 60. Mitochondrial function, as well as superoxide content, were decreased in most brain areas on PND0 and went through adaptation, showing increased mass and membrane potential in adulthood. Concerning mitochondrial electron transport system (METS), the most affected area was the cerebellum, which was impaired at birth and activated at adulthood. In conclusion, our results show that gestational CR promotes adaptation from impaired mitochondrial parameters at birth, improving mitochondrial function when compared to control, without increasing superoxide generation, at adult age. More studies are necessary in order to support the use of CR as a clinical approach.

中文翻译：

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妊娠热量限制对 Wistar 大鼠大脑线粒体的适应性影响：一种 DOHaD 方法

健康与疾病的发育起源 (DOHaD) 是一个生物科学领域，致力于研究发育过程中的不同干预措施如何影响个体的生活。饮食是与环境互动的重要方式，怀孕期间不仅会影响母亲，还会影响下一代。这些干预措施之一是热量限制 (CR)，当营养不良得到负责任的控制时，它在大鼠后代中显示出积极的氧化还原调节。考虑到线粒体代谢是氧化还原状态的决定因素，我们研究了与线粒体功能和后代大脑中反应性物种水平相关的参数，这些参数来自被送到怀孕热量限制大坝的大鼠。所以，怀孕大鼠分为对照组（随意进食）和 CR（20% 食物限制加微量营养素补充）组，并在出生后第 0、7、21 和 60 天分析后代的大脑。线粒体功能，如以及超氧化物含量，在 PND0 的大多数大脑区域中都减少并经历了适应，在成年期显示出增加的质量和膜电位。关于线粒体电子传递系统 (METS)，受影响最严重的区域是小脑，它在出生时受损并在成年时被激活。总之，我们的结果表明，妊娠 CR 促进了出生时线粒体参数受损的适应，与对照相比，在成年时改善了线粒体功能，而不会增加超氧化物的产生。