Abstract

We studied the effect of zinc excess (1000 μM) and low positive temperature (4°C) on the IRT1 gene expression in barley roots and leaves. Exposure at each of the stress factors separately induced an increase in the content of the HvIRT1 gene transcripts, which was more pronounced in leaves. At the same time, the growth of seedlings continued. Under the combined action of the stress factors in the first 3 days, the amount of mRNA also increased, but after 7 days of exposure it significantly declined, which correlated with the complete inhibition of seedling growth. It is assumed that the seedling growth inhibition under the combined effect of zinc excess and low temperature is associated with a decrease in the transcriptional activity of the HvIRT1 gene, due to the deficiency in a number of trace elements under these conditions.

中文翻译：

---

锌过量和低温对大麦根和叶片IRT1基因表达的影响。

摘要

我们研究了过量的锌（1000μM）和较低的正温度（4°C）对大麦根和叶中IRT1基因表达的影响。在每种胁迫因子下的暴露分别诱导HvIRT1基因转录物含量的增加，这在叶片中更为明显。同时，幼苗的生长持续。在胁迫因子的前三天的共同作用下，mRNA的量也增加了，但是在暴露7天后，其量显着下降，这与幼苗生长的完全抑制有关。推测在锌过量和低温共同作用下的幼苗生长抑制与HvIRT1转录活性的降低有关 基因，由于在这些条件下许多微量元素的缺乏。