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Generation of a novel mouse strain with conditional, cell-type specific, expression of DND1.
genesis ( IF 1.5 ) Pub Date : 2019-09-12 , DOI: 10.1002/dvg.23335
Lisa Nunez 1 , Sharada Mokkapati 2 , Chengtai Yu 3 , Jian M Deng 2 , Richard R Behringer 2 , Angabin Matin 1
Affiliation  

Dead‐End 1 (DND1) encodes an RNA binding protein critical for viable primordial germ cells in vertebrates. When introduced into cancer cell lines, DND1 suppresses cell proliferation and enhances apoptosis. However, the molecular function of mammalian wild‐type DND1 has mostly been studied in cell lines and not verified in the organism. To facilitate study of wild‐type DND1 function in mammalian systems, we generated a novel transgenic mouse line, LSL‐FM‐DND1 flox/+, which conditionally expresses genetically engineered, FLAG‐tagged and myc‐tagged DND1 in a cell type‐specific manner. We report that FLAG‐myc‐DND1 is indeed expressed in specific tissues of the mouse when LSL‐FM‐DND1 flox/+ is combined with mouse strains expressing Cre‐recombinase. LSL‐FM‐DND1 flox/+ mice are fertile with no overt health effects. We expressed FLAG‐myc‐DND1 in the pancreas and found that chronic, ectopic expression of FLAG‐myc‐DND1 led to increase in fasting glucose levels in older mice. Thus, this novel LSL‐FM‐DND1 flox/+ mouse strain will facilitate studies on the biological and molecular function of wild‐type DND1.

中文翻译:

产生具有DND1条件性细胞类型特异性表达的新型小鼠品系。

Dead-End 1(DND1)编码一种RNA结合蛋白,对脊椎动物的存活原始生殖细胞至关重要。当导入癌细胞系时,DND1抑制细胞增殖并增强凋亡。但是,哺乳动物野生型DND1的分子功能主要是在细胞系中研究的,尚未在生物体中得到验证。为了促进对哺乳动物系统中野生型DND1功能的研究,我们生成了一种新型转基因小鼠品系LSL-FM-DND1 flox / +,可在特定于细胞类型的细胞中有条件地表达经过基因工程改造,带有FLAG标签和myc标签的DND1方式。我们报告说,当LSL-FM-DND1 flox / +与表达Cre-recombinase的小鼠品系组合时,FLAG-myc-DND1确实在小鼠的特定组织中表达 LSL-FM-DND1 flox / +小鼠可育,没有明显的健康影响。我们在胰腺中表达了FLAG-myc-DND1,发现FLAG-myc-DND1的慢性异位表达导致老年小鼠的空腹血糖水平升高。因此,这种新颖的LSL-FM-DND1 flox / +小鼠品系将有助于研究野生型DND1的生物学和分子功能。
更新日期:2019-09-12
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