Caffeic acid is found in variety of fruits and vegetables. It is considered as possible human carcinogen (Group 2B). It is negative in Ames and mouse micronucleus (MN), but positive in mouse lymphoma and chromosomal aberration assays. The objective of this study was to evaluate the in vivo genotoxicity of caffeic acid using three different endpoints: in vivo MN, Pig-a, and comet assay. Two sets of six rats per group were administered vehicle (0.5% hydroxypropyl methylcellulose), 500, 1,000, or 2,000 mg/kg/day of caffeic acid for three consecutive days via oral gavage. One set of animals was used for the Pig-a and MN assay and the other set was used for the comet assay. N-Ethyl N-Nitrosourea was used as positive control for the Pig-a and MN assay, and ethyl methanesulfonate for the comet assay. From one set of animals, peripheral blood was collected on Days -1, 14, and 30 for the Pig-a assay and on Day 4 for the MN assay. The other set of animals was euthanized 3 hr after the last dose; liver and blood were collected for the comet assay. A statistically significant increase in the MN frequency was observed at 2,000 mg/kg/day. No increase in the red blood cells (RBCCD59- ) or reticulocytes (RETCD59- ) Pig-a mutant frequencies was observed on Days 14 or 30. No increase in DNA strand breaks was observed in the peripheral blood or liver in the comet assay. Environ. Mol. Mutagen. 2019. © 2019 Wiley Periodicals, Inc.

中文翻译：

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咖啡酸的遗传毒性：猪a分析与体内遗传遗传毒理学的相关性。

咖啡酸存在于各种水果和蔬菜中。它被认为是可能的人类致癌物（2B族）。在Ames和小鼠微核（MN）中为阴性，在小鼠淋巴瘤和染色体畸变分析中为阳性。这项研究的目的是使用三种不同的终点评估咖啡酸的体内遗传毒性：体内MN，Pig-a和彗星试验。通过口服管饲法连续三天给每组的两组六只大鼠施用媒介物（0.5％羟丙基甲基纤维素），500、1,000或2,000 mg / kg /天的咖啡酸。一组动物用于Pig-a和MN测定，另一组动物用于彗星测定。N-乙基N-亚硝基脲用作Pig-a和MN测定的阳性对照，甲磺酸乙酯用于彗星测定。从一组动物中 在第-1、14和30天收集外周血用于Pig-a分析，在第4天收集MN分析。在最后一次给药后3小时将另一组动物安乐死；收集肝脏和血液用于彗星试验。在2,000 mg / kg /天时，观察到MN频率有统计学上的显着增加。在第14天或第30天，未观察到红细胞（RBCCD59-）或网织红细胞（RETCD59-）Pig-a突变体的频率增加。在彗星试验中，在外周血或肝脏中未观察到DNA链断裂的增加。环境。大声笑 诱变剂。2019.©2019 Wiley Periodicals，Inc. 在2,000 mg / kg /天时，观察到MN频率有统计学上的显着增加。在第14天或第30天，未观察到红细胞（RBCCD59-）或网织红细胞（RETCD59-）Pig-a突变体的频率增加。在彗星试验中，在外周血或肝脏中未观察到DNA链断裂的增加。环境。大声笑 诱变剂。2019.©2019 Wiley Periodicals，Inc. 在2,000 mg / kg /天时，观察到MN频率有统计学上的显着增加。在第14天或第30天，未观察到红细胞（RBCCD59-）或网织红细胞（RETCD59-）Pig-a突变体的频率增加。在彗星试验中，在外周血或肝脏中未观察到DNA链断裂的增加。环境。大声笑 诱变剂。2019.©2019 Wiley Periodicals，Inc.