Tetrahydrofuran (THF) is a ubiquitous toxic and carcinogenic pollutant. Screening for pure or mixed-culture microorganisms that can efficiently degrade THF is difficult due to its chemical stability. In this study, an enrichment culture, H-1, with a stable THF-degrading ability and microbial community structure was enriched from activated sludge and could efficiently degrade 95% of 40 mM THF within 6 days. The optimal THF degradation conditions for H-1 were an initial pH of 7.0–8.0 and a temperature of 30 °C. The substrate tolerance concentration of H-1 reached 200 mM. Heavy metals tolerance concentrations of Cu²⁺, Cd²⁺ and Pb²⁺ of H-1 was 0.5 mM, 0.4 mM and 0.03 mM, and 4 mM Mn²⁺ did not significantly influence the THF degradation ratio or biomass of H-1. H-1 might be a good material for actual wastewater treatment because of its efficient THF degradation performance and ability to resist various stressful conditions. In addition, the THF-degrading efficiency of H-1 was enhanced by the addition of moderate carbon sources. High-throughput sequencing of the 16S rRNA gene showed that Rhodococcus sp. (a potential THF-degrading strain) and Hydrogenophaga sp. (a potential non-THF-degrading strain) were the dominant microorganisms in the H-1 culture. These results indicate the potential coexistence of cooperation and competition between THF-degrading bacteria and nondegrading bacteria in this enrichment culture.

中文翻译：

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高效四氢呋喃降解细菌培养物的富集和表征。

四氢呋喃（THF）是一种普遍存在的有毒致癌污染物。由于其化学稳定性，难以筛选可有效降解THF的纯或混合培养微生物。在这项研究中，从污泥中富集具有稳定的THF降解能力和微生物群落结构的H-1富集培养物，可以在6天内有效降解95％的40 mM THF。H-1的最佳THF降解条件是初始pH值为7.0-8.0，温度为30°C。H-1的底物耐受浓度达到200 mM。H-1的Cu 2 ⁺，Cd ²⁺和Pb ²⁺的重金属耐受浓度为0.5 mM，0.4 mM和0.03 mM，以及4 mM Mn ²⁺不会明显影响H-1的THF降解率或生物量。H-1由于其有效的THF降解性能和抵抗各种压力条件的能力，可能是用于实际废水处理的良好材料。此外，通过添加适度的碳源可以提高H-1的THF降解效率。16S rRNA基因的高通量测序表明，红球菌属。（潜在的THF降解菌株）和Hydrogenophaga sp。（潜在的非THF降解菌株）是H-1培养物中的优势微生物。这些结果表明在这种富集培养中，THF降解细菌和非降解细菌之间可能存在合作与竞争。