Ortho-phenylphenol (OPP), as an active ingredient of disinfectants, has been worldwide utilized as fungicides and antibacterial agents in hospital, agriculture, wood preservation, and veterinary products. However, little is known about the toxic effects of OPP on male reproduction, especially sperm motility, and the underlying mechanisms. In this study, we chose porcine sperms as in vitro model to investigate the effects and mechanisms of OPP exposure on sperm motility. Our results indicated that porcine sperm motility decreases significantly in a dose-dependent manner after exposed to OPP. Additionally, ATP synthesis deficiency was revealed by downregulation of ATP synthase subunit beta and adenosine 5'-monophosphate-activated protein kinase expression. Furthermore, OPP disturbed the expression of TP53 and PTEN, which contributed to AKT pathway deactivation. OPP exposure also disrupted platelet-derived growth factor receptor A expression, which further inhibited 3-phosphoinositide-dependent protein kinase 1 activation, resulting in protein kinase B and pyruvate dehydrogenase phosphatase catalytic subunit 1 deactivation. In conclusion, these observations suggest that OPP exposure decreases porcine sperm motility by disturbing the AMPK/AKT signaling pathway. Environ. Mol. Mutagen. 2019. © 2019 Wiley Periodicals, Inc.

中文翻译：

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邻苯酚暴露会通过AMPK / AKT信号传导途径损害猪精子活力。

邻苯酚（OPP）作为消毒剂的活性成分，已在医院，农业，木材防腐和兽医产品中用作杀菌剂和抗菌剂。然而，关于OPP对雄性生殖，特别是精子运动性的毒性作用以及潜在的机制知之甚少。在这项研究中，我们选择猪精子作为体外模型，以研究OPP暴露对精子活力的影响及其机制。我们的结果表明，暴露于OPP后，猪精子活力以剂量依赖性方式显着降低。此外，ATP合成酶亚基β和腺苷5'-单磷酸激活的蛋白激酶表达下调揭示了ATP合成缺陷。此外，OPP干扰了TP53和PTEN的表达，导致AKT通路失活。OPP暴露还破坏了血小板衍生的生长因子受体A的表达，从而进一步抑制了3-磷酸肌醇依赖性蛋白激酶1的激活，从而导致蛋白激酶B和丙酮酸脱氢酶磷酸酶催化的亚基1失活。总之，这些观察结果表明，OPP暴露会通过干扰AMPK / AKT信号传导途径而降低猪精子活力。环境。大声笑 诱变剂。2019.©2019 Wiley Periodicals，Inc. 这些观察结果表明，OPP暴露会通过干扰AMPK / AKT信号传导途径而降低猪精子活力。环境。大声笑 诱变剂。2019.©2019 Wiley Periodicals，Inc. 这些观察结果表明，OPP暴露会通过干扰AMPK / AKT信号传导途径而降低猪精子活力。环境。大声笑 诱变剂。2019.©2019 Wiley Periodicals，Inc.