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The insect-protected CTC91087-6 sugarcane event expresses Cry1Ac protein preferentially in leaves and presents compositional equivalence to conventional sugarcane.
GM Crops & Food ( IF 3.9 ) Pub Date : 2019-08-20 , DOI: 10.1080/21645698.2019.1651191
Adriana C Gianotto 1 , Moisés S Rocha 1 , Lucas Cutri 1 , Francisco C Lopes 1 , William Dal'Acqua 1 , Jerry J Hjelle 2 , Ron P Lirette 3 , Wladecir S Oliveira 1 , Maria L Sereno 1
Affiliation  

A Cry1Ac-expressing sugarcane cultivar, CTC91087-6, has been developed by Centro de Tecnologia Canavieira (CTC) to be resistant to the sugarcane borer (Diatraea saccharalis). This genetically modified event was developed using Agrobacterium-mediated transformation and the help of the selectable marker phosphinothricin N-acetyltransferase (PAT) expressed from bar gene. We describe here a detailed characterization of CTC91087-6 event with respect to protein expression, nutritional composition, and assessment of its derived DNA and proteins in raw sugar. Expression of the Cry1Ac and PAT (bar) proteins produced by CTC91087-6 was evaluated in different tissues and at different times during the growing season. The new proteins are preferentially expressed in leaves, are produced at low levels in stalks, and are near the limits of detection in root tissues. The levels of Cry1Ac were much higher than PAT in all evaluated tissues. Furthermore, Cry1Ac levels in CTC91087-6 leaves are stable at various times during sugarcane cultivation cycle, assuring borer control throughout the complete crop cycle. Assessment of CTC91087-6 tissues for key food and feed nutrients as recommended by OECD to assess the safety of new varieties of sugarcane showed compositional equivalence to the conventional counterpart CTC9001 and to other commercial sugarcane varieties used as references. Raw sugar samples produced from CTC91087-6 did not contain DNA corresponding to cry1Ac and bar genes nor DNA specifically derived from CTC91087-6. In a similar way, there is no detection of Cry1Ac and PAT proteins in raw sugar produced from CTC91087-6. Taken together these results show that CTC91087-6 stably expresses Cry1Ac and PAT proteins and is substantially equivalent to the conventional counterpart CTC9001.

中文翻译:

昆虫保护的CTC91087-6甘蔗事件在叶片中优先表达Cry1Ac蛋白,并在成分上与常规甘蔗相当。

一种表达Cry1Ac的甘蔗品种,CTC91087-6,由Centro de Tecnologia Canavieira(CTC)开发,可以抵抗甘蔗bore(Diatraea saccharalis)。利用农杆菌介导的转化和从bar基因表达的选择性标记膦丝菌素N-乙酰基转移酶(PAT)的帮助,开发了这种基因修饰事件。我们在这里描述CTC91087-6事件的详细特征,涉及蛋白质表达,营养成分以及对原糖中其衍生DNA和蛋白质的评估。在生长季节的不同组织和不同时间评估了CTC91087-6产生的Cry1Ac和PAT(bar)蛋白的表达。新蛋白质优先在叶片中表达,在茎中含量低,并且接近于在根组织中的检测极限。在所有评估的组织中,Cry1Ac的水平均远高于PAT。此外,CTC91087-6叶片中Cry1Ac的水平在甘蔗栽培周期的各个时间均保持稳定,从而确保了整个作物周期中的蛀虫控制。经合组织建议评估CTC91087-6组织的主要食品和饲料营养成分,以评估甘蔗新品种的安全性,其成分等同于常规对等CTC9001和其他商业甘蔗品种作为参考。从CTC91087-6生产的原糖样品不包含与cry1Ac和bar基因相对应的DNA,也不包含专门从CTC91087-6衍生的DNA。以类似的方式,在CTC91087-6生产的原糖中没有检测到Cry1Ac和PAT蛋白。
更新日期:2019-11-01
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