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Tris(1,3-dichloro-2-propyl) phosphate disturbs mouse embryonic development by inducing apoptosis and abnormal DNA methylation.
Environmental and Molecular Mutagenesis ( IF 2.8 ) Pub Date : 2019-08-30 , DOI: 10.1002/em.22322
Shu-Yuan Yin 1, 2 , Li Chen 1, 2 , Dan-Ya Wu 1, 2 , Tao Wang 1, 2 , Li-Jun Huo 2 , Shuhong Zhao 2 , Jilong Zhou 1, 2 , Xia Zhang 1, 3 , Yi-Liang Miao 1, 2, 4
Affiliation  

Tris(1,3-dichloro-2-propyl) phosphate (TDCPP) is a kind of additive flame retardants (FRs) and was found to affect early embryonic development in zebrafish; however, there are few studies to investigate whether TDCPP will disturb the development of early mouse embryos. In our studies, we used mouse embryos as models to study the toxicology of TDCPP on the early embryos. The results showed that TDCPP disturbed the development of early mouse embryos in a dose-dependent manner. 10 μM TDCPP decreased the blastocyst formation and 100 μM TDCPP was a lethal concentration for the mouse embryos. We proved that TDCPP was detrimental to embryonic development potential by increasing the reactive oxygen species level and inducing early apoptosis. Furthermore, TDCPP changed the DNA methylation patterns of imprinted genes in treated blastocysts. The methylation of H19 and Snrpn promoter regions was increased from 37.67% to 46.00% and 31.56% to 44.38% in treated groups, respectively. In contrast, Peg3 promoter region methylation was declined from 86.55% to 73.27% in treated embryos. Taken together, our results demonstrated that TDCPP could adversely impair the early embryonic development in mouse. Environ. Mol. Mutagen. 2019. © 2019 Wiley Periodicals, Inc.

中文翻译:

磷酸三(1,3-二氯-2-丙基)酯通过诱导细胞凋亡和异常DNA甲基化干扰小鼠胚胎发育。

磷酸三(1,3-二氯-2-丙基)酯(TDCPP)是一种添加剂阻燃剂(FRs),被发现会影响斑马鱼的早期胚胎发育。但是,很少有研究来研究TDCPP是否会干扰早期小鼠胚胎的发育。在我们的研究中,我们使用小鼠胚胎作为模型来研究TDCPP对早期胚胎的毒理学。结果表明,TDCPP以剂量依赖的方式干扰了早期小鼠胚胎的发育。10μMTDCPP减少了胚泡的形成,而100μMTDCPP对小鼠胚胎而言是致死浓度。我们证明了TDCPP通过增加活性氧水平并诱导早期凋亡而有害于胚胎发育。此外,TDCPP改变了处理过的胚泡中印迹基因的DNA甲基化模式。在治疗组中,H19和Snrpn启动子区域的甲基化分别从37.67%增加到46.00%,从31.56%增加到44.38%。相反,在处理过的胚胎中,Peg3启动子区域甲基化从86.55%下降到73.27%。两者合计,我们的结果表明TDCPP可能不利地损害小鼠的早期胚胎发育。环境。大声笑 诱变剂。2019.©2019 Wiley Periodicals,Inc.
更新日期:2019-11-01
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