Aerobic granular sludge (AGS) technology is generally negatively affected by the salinity in high saline organic wastewater. The effect of salinity on organic pollutants removal of AGS was studied in three parallel sequencing batch reactors. The results indicated that the performance of reactors operating at relative low salinity (1%) remained stable. However, at medium salinity (2%) and higher salinity (4%) conditions, the organic pollutants removal efficiencies deteriorated from 93.7 ± 3.0 to 71.6 ± 6.8 and 53.6 ± 5.4%, respectively. The addition of a mixture of acyl-homoserine lactone (AHL) mediated quorum sensing (QS) signaling molecules (0.1 μmol/L of mixed C6-HSL, C8-HSL and 3OC8-HSL) only restored the performance of the 2% salinity reactor back to 86.3 ± 6.2% due to the changing of hydrophobic extracellular polymeric substance ratio from 64 ± 3 to 71 ± 4%. Addition of the AHL had no effect on the pollution removal efficiency at the 4% salinity conditions. Microbial community analysis showed that Dyella (32.3%) species were the dominant member of the community and its occurrence was positively correlated with organic pollutants removal efficiency at relative high salinity (2% and 4%), while Mangrovibacter showed the opposite trend. Higher abundances of hdtS and acylase genes, the synthesis and degradation genes of AHL, were found after adding AHLs to reactors at 2% salinity, which indicated that AHL mediated QS was the primary QS system in salt-tolerant AGS.

中文翻译：

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群体感应对耐盐需氧颗粒污泥性能的影响：将细胞外聚合物与微生物群落联系起来。

好氧颗粒污泥（AGS）技术通常受到高盐分有机废水中盐度的负面影响。在三个平行顺序分批反应器中研究了盐度对AGS去除有机污染物的影响。结果表明，在较低盐度（1％）下运行的反应堆性能保持稳定。但是，在中等盐度（2％）和较高盐度（4％）的条件下，有机污染物的去除效率分别从93.7±3.0降至71.6±6.8和53.6±5.4％。加入酰基高丝氨酸内酯（AHL）介导的群体感应（QS）信号分子的混合物（0.1μmol/ L的混合C6-HSL，C8-HSL和3OC8-HSL）只能恢复2％盐度反应器的性能回到86.3±6。2％是由于疏水性细胞外聚合物质比率从64±3变为71±4％。在4％的盐度条件下，添加AHL对去除污染物的效率没有影响。微生物群落分析表明在相对较高的盐度下（2％和4％），Dyella（32.3％）种是该群落的主要成员，其发生与有机污染物的去除效率呈正相关，而Mangrovibacter则呈现相反的趋势。在盐度为2％的反应器中添加AHL后，发现了较高的hdtS和酰基转移酶基因，AHL的合成和降解基因，这表明AHL介导的QS是耐盐AGS中的主要QS系统。