The high rate of cell proliferation in the zebrafish brain allows one to perform reliable studies on cellular molecules associated with cell proliferation. In this study, we show that 5-bromo-2'-deoxyuridine (BrdU)-labeled cells were detected in the isolated brains of adult zebrafish that were incubated in oxygenated artificial cerebrospinal fluid (aCSF) in vitro. The number of BrdU-labeled cells shown in the in vitro preparation was close to that in the in vivo study and was modified by treatment with various pharmacological agents, such as valproic acid. In parallel to cell proliferation, protein expression and neural activity could also be measured in the isolated brains maintained in aCSF.

中文翻译：

---

在人工脑脊髓液中孵育的成年斑马鱼离体大脑中的细胞增殖。

斑马鱼大脑中的高细胞增殖率使人们能够对与细胞增殖有关的细胞分子进行可靠的研究。在这项研究中，我们表明，在成年斑马鱼的分离脑中检测到了5-溴2'-脱氧尿苷（BrdU）标记的细胞，这些脑在体外含氧人工脑脊髓液（aCSF）中孵育。体外制剂中显示的BrdU标记的细胞数量与体内研究中的数量接近，并通过用各种药理学药物（如丙戊酸）进行了修饰。与细胞增殖并行，还可以在aCSF中维持的孤立大脑中测量蛋白质表达和神经活动。