Pseudocapillaria tomentosa is an important pathogen in zebrafish facilities. We investigated heat, ultraviolet (UV) light, chlorine, iodine, and dessciation for killing the parasite's eggs. Eggs released with feces larvate in about 5-10 days, and treatments were evaluated by exposing fresh eggs and subsequently comparing larvation to untreated eggs as an indication of survival. Collectively, untreated eggs in all trials showed high levels of survival. Eggs were exposed to elevated temperatures (40°C, 45°C and 50°C) for 1, 8, or 24 h, which resulted in substantial reduction in viability of eggs. UV radiation was effective, with no larvation at 50-300 mWs/cm2 and <2% at 20 mWs/cm2. Three chlorine products (JT Baker, Clorox®, and Bi-Mart) were tested at 25, 50, 100, 500, and 3,000 ppm (pH 7.0-7.3) with 10 min exposure. All were effective at 500 or 1,000 ppm. There was variability between three products and trials at lower concentrations, but overall chlorine was not very effective at 25-100 ppm except for Bi-Mart brand at 100 ppm. Povidone-iodine was not effective at 25 or 50 ppm for 10 min, but was effective at 200 ppm for 1 h. Desiccation was effective, and no eggs larvated after 2 h drying.

中文翻译：

---

暴露于热，紫外线，氯，碘和干燥下的毛细假单胞菌卵的生存力。

假毛细血管假单胞菌是斑马鱼设施中的重要病原体。我们调查了热，紫外线（UV），氯，碘和干燥剂对杀死寄生虫卵的影响。粪便中释放的卵大约在5-10天后幼虫释放，然后通过暴露新鲜卵，然后将幼虫与未处理的卵进行比较来评估其存活率，以评估治疗效果。总的来说，所有试验中未处理的卵均显示出较高的存活率。鸡蛋暴露于高温（40°C，45°C和50°C）下1、8或24小时，这导致鸡蛋的生存能力大大降低。紫外线辐射是有效的，在50-300 mWs / cm2时没有幼虫，在20 mWs / cm2时不到2％。在25、50、100、500和3,000 ppm（pH 7.0-7.3）下测试了三种氯气产品（JT Baker，Clorox®和Bi-Mart），暴露时间均为10分钟。所有这些都在500或1,000 ppm时有效。三种产品和较低浓度的试验之间存在差异，但总的氯浓度在25-100 ppm时不是很有效，除了Bi-Mart品牌在100 ppm时。聚维酮碘在25或50 ppm下无效10分钟，但在200 ppm下有效1 h。干燥是有效的，干燥2小时后没有卵幼虫。