One of the major functions of the protein homeostasis system is to maintain proteins in their soluble states, and indeed several human disorders are associated with the aberrant aggregation of proteins. An active involvement of the protein homeostasis system is necessary to avoid aggregation because proteins are expressed at levels close to their solubility limits, hence being poorly soluble. The mechanisms by which the protein homeostasis system acts to control protein aggregation are, however, still not known in much detail. To facilitate systematic investigations of these mechanisms, we describe here the CamSol method of predicting protein solubility, and illustrate its initial applications. We anticipate that with the advent of powerful proteomics and transcriptomic methods, in combination with the use of the CamSol method and related approaches to predict the solubility and other biophysical properties of proteins, it will become possible to increase our understanding of the principles of protein homeostasis related to the maintenance of the proteome in its soluble form.

中文翻译：

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在蛋白质稳态研究中使用 CamSol 方法预测蛋白质溶解度。

蛋白质稳态系统的主要功能之一是将蛋白质维持在其可溶状态，事实上，几种人类疾病与蛋白质的异常聚集有关。蛋白质稳态系统的积极参与对于避免聚集是必要的，因为蛋白质的表达水平接近其溶解度极限，因此溶解性差。然而，蛋白质稳态系统控制蛋白质聚集的机制尚不清楚。为了促进对这些机制的系统研究，我们在这里描述了预测蛋白质溶解度的 CamSol 方法，并说明了它的初始应用。我们预计，随着强大的蛋白质组学和转录组学方法的出现，