We cloned eight transcription factors that activate lavender monoterpene synthase promoters. In this study, we employed the Yeast One-Hybrid (Y1H) assay system to identify transcription factors that control promoters for two Lavandula × intermedia monoterpene synthase genes, linalool synthase (LiLINS) and 1,8-cineole synthase (LiCINS). The bait sequences used in the assay were either a 768-bp LiLINS, or a 1087-bp LiCINS promoter. The prey included proteins expressed in L. × intermedia floral tissue. The assay identified 96 sequences encoding proteins that interacted with one or both promoters. To explore the nature of this interaction, the LiLINS and LiCINS promoter fragments were each fused to the E. coli gusA (GUS) reporter gene. The constructs were separately transformed into tobacco (Nicotiana benthamiana) leaves co-expressing individually a subset of ten representative transcription factors (TFs) predicted to control these promoters. Six TFs induced expression from both promoters, two activated LiCINS promoter alone, and two did not induce expression from either promoter. The TFs identified in this study belong to various groups including those containing conserved domains typical of MYB, bZIP, NAC, GeBP and SBP-related proteins.

中文翻译：

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多种转录因子控制薰衣草 (Lavandula) 中单萜合酶的表达

我们克隆了八种激活薰衣草单萜合酶启动子的转录因子。在这项研究中，我们使用酵母一杂交 (Y1H) 测定系统来鉴定控制两个薰衣草 × 中间单萜合酶基因、芳樟醇合酶 (LiLINS) 和 1,8-桉叶素合酶 (LiCINS) 的启动子的转录因子。测定中使用的诱饵序列是 768-bp LiLINS 或 1087-bp LiCINS 启动子。猎物包括在 L. × intermedia 花组织中表达的蛋白质。该测定鉴定了 96 个编码与一个或两个启动子相互作用的蛋白质的序列。为了探索这种相互作用的性质，将 LiLINS 和 LiCINS 启动子片段分别融合到大肠杆菌 gusA (GUS) 报告基因上。将构建体分别转化到烟草 (Nicotianabenthamiana) 叶子中，分别共表达预测控制这些启动子的 10 个代表性转录因子 (TF) 的子集。六个 TF 诱导两个启动子的表达，两个单独激活的 LiCINS 启动子，两个不诱导任一启动子的表达。本研究中鉴定的 TF 属于不同的组，包括那些包含 MYB、bZIP、NAC、GeBP 和 SBP 相关蛋白典型的保守结构域的组。