Molecular farming increasingly uses the tobacco relative Nicotiana benthamiana for production of recombinant proteins through transient expression. Several proteins are produced efficiently with this expression platform, but yields for other proteins are often very low. These low yields are frequently due to endogenous proteases. The latest genome annotations indicate that N. benthamiana encodes for at least 1243 putative proteases that probably act redundantly and consecutively on substrates in different subcellular compartments. Here, we discuss the N. benthamiana protease repertoire that may affect recombinant protein production and recent advances in protease depletion strategies to increase recombinant protein production in N. benthamiana.

中文翻译：

---

烟草：本氏烟草的蛋白酶：分子农业的新战役。

分子农业越来越多地使用烟草亲本烟草本氏菌通过瞬时表达来生产重组蛋白。使用该表达平台可以有效地生产几种蛋白质，但是其他蛋白质的产量通常非常低。这些低产量通常归因于内源蛋白酶。最新的基因组注释表明，本氏烟草编码至少1243种假定的蛋白酶，这些蛋白酶可能在不同亚细胞区室中的底物上连续冗余地起作用。在这里，我们讨论可能影响重组蛋白生产的本氏烟草的蛋白酶库，以及增加本氏烟草中重组蛋白产量的蛋白酶耗竭策略的最新进展。