In periodontitis, polymorphonuclear leucocytes (PMNs) are activated. They entrap and eliminate pathogens by releasing neutrophil extracellular traps (NETs). Abnormal NET degradation is part of a pro-inflammatory status, affecting co-morbidities such as cardiovascular disease. We aimed to investigate the ex vivo NET degradation capacity of plasma from periodontitis patients compared to controls (part 1) and to quantify NET degradation before and after periodontal therapy (part 2). Fresh NETs were obtained by stimulating blood-derived PMNs with phorbol 12-myristate 13-acetate. Plasma samples from untreated periodontitis patients and controls were incubated for 3 h onto freshly generated NETs (part 1). Similarly, for part 2, NET degradation was studied for 91 patients before and 3, 6 and 12 mo after non-surgical periodontal therapy with and without adjunctive systemic antibiotics. Finally, NET degradation was fluorospectrometrically quantified. NET degradation levels did not differ between periodontitis patients and controls, irrespective of subject-related background characteristics. NET degradation significantly increased from 65.6 ± 1.7% before periodontal treatment to 75.7 ± 1.2% at 3 mo post periodontal therapy, and this improvement was maintained at 6 and 12 mo, irrespective of systemic usage of antibiotics. Improved NET degradation after periodontitis treatment is another systemic biomarker reflecting a decreased pro-inflammatory status, which also contributes to an improved cardiovascular condition.

中文翻译：

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牙周治疗会增加中性粒细胞胞外陷阱的降解。

在牙周炎中，多形核白细胞（PMN）被激活。它们通过释放中性粒细胞胞外陷阱（NET）来捕获并消除病原体。异常的 NET 降解是促炎症状态的一部分，会影响心血管疾病等并发症。我们的目的是研究与对照组相比，牙周炎患者血浆的离体 NET 降解能力（第 1 部分），并量化牙周治疗前后的 NET 降解（第 2 部分）。新鲜的 NET 是通过用佛波醇 12-肉豆蔻酸酯 13-乙酸酯刺激血液来源的 PMN 获得的。将未经治疗的牙周炎患者和对照的血浆样本在新生成的 NET 上孵育 3 小时（第 1 部分）。同样，第 2 部分对 91 名患者进行了非手术牙周治疗前以及非手术牙周治疗后 3、6 和 12 个月（使用或不使用辅助全身抗生素）的 NET 降解研究。最后，对 NET 降解进行荧光光谱定量。无论受试者相关的背景特征如何，牙周炎患者和对照组之间的 NET 降解水平没有差异。NET 降解从牙周治疗前的 65.6 ± 1.7% 显着增加至牙周治疗后 3 个月的 75.7 ± 1.2%，并且无论全身使用抗生素如何，这种改善在 6 个月和 12 个月时都保持不变。牙周炎治疗后 NET 降解的改善是另一个全身生物标志物，反映了促炎状态的降低，这也有助于改善心血管状况。