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A single-cell view of tissue regeneration in plants.
Current Opinion in Plant Biology ( IF 9.5 ) Pub Date : 2019-10-23 , DOI: 10.1016/j.pbi.2019.09.003
Victoria Mironova 1 , Jian Xu 2
Affiliation  

With the development of advanced molecular-genetic and computational technologies it becomes possible to tackle individual cells within a regenerating tissue, to define morphogenetic and cellular changes in space and time by live imaging, to acquire transcriptome status with single-cell RNA sequencing (ScRNA-seq), and to delineate the candidate mechanisms by iterative computational and experimental approaches. Here, we review recent findings and current knowledge on tissue regeneration in plants, focusing on four evolutionarily conserved scenarios that a cell may embark on to facilitate the regeneration of a plant tissue structure lost by injury, namely cell death, division, dedifferentiation, and transdifferentiation. Understanding of these scenarios at single-cell resolution, singularly and in combination, could provide an unprecedented view of tissue regeneration in plants.

中文翻译:

植物组织再生的单细胞视图。

随着先进的分子遗传学和计算技术的发展,有可能解决再生组织中的单个细胞,通过实时成像来定义空间和时间上的形态发生和细胞变化,并通过单细胞RNA测序获得转录组状态(ScRNA- seq),并通过迭代的计算和实验方法来描述候选机制。在这里,我们回顾了有关植物组织再生的最新发现和当前知识,重点介绍了细胞可能着手促进因损伤而丧失的植物组织结构的再生的四种进化保守场景,即细胞死亡,分裂,去分化和转分化。 。以单细胞分辨率或组合方式了解这些场景,
更新日期:2019-10-23
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