CRISPR/Cas is in the process of inducing the biggest transformation of plant breeding since the green revolution. Whereas initial efforts focused mainly on changing single traits by error prone non-homologous end joining, the last two years saw a tremendous technical progress achieving more complex genetic, epigenetic and transcriptional changes. The efficiencies of inducing directed changes by homologous recombination have been improved significantly and strategies to break genetic linkages by inducing chromosomal rearrangements have been developed. Cas13 systems have been applied to degrade viral and mRNA in plants. Most importantly, a historical breakthrough was accomplished: By introducing multiple genomic changes simultaneously, domestication of wild species in a single generation has been demonstrated, speeding up breeding dramatically.

中文翻译：

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CRISPR / Cas将植物生物学和育种带入了快速通道。

自绿色革命以来，CRISPR / Cas正在诱导最大的植物育种转化。最初的努力主要集中在通过容易出错的非同源末端连接来改变单个性状，而最近两年看到了巨大的技术进步，实现了更复杂的遗传，表观遗传和转录变化。通过同源重组诱导定向改变的效率已得到显着提高，并且已经开发了通过诱导染色体重排破坏遗传联系的策略。Cas13系统已应用于降解植物中的病毒和mRNA。最重要的是，实现了历史性突破：通过同时引入多种基因组变化，已经证明了单代野生物种的驯化，极大地加快了繁殖速度。