Mutations in the X-linked phosphatidylinositol glycan, class A gene (Pig-a) lead to loss of glycosylphosphatidylinositol (GPI) anchors and GPI-anchored proteins from the surface of erythrocytes and other mammalian cells. The Pig-a gene mutation assay quantifies in vivo gene mutation by immunofluorescent labeling and flow cytometry to detect the loss of GPI-anchored proteins on peripheral blood erythrocytes. As part of the regulatory acceptance of the assay, a public database has been created that provides detailed information on Pig-a gene mutation assays conducted in rats and mice. A searchable version of the database is available through a website designed and hosted by the University of Maryland School of Pharmacy. Currently, the database contains only mouse and rat data, but it is anticipated that it will expand to include data from other species, including humans. A major purpose in developing the database was to aid in the preparation of a Retrospective Performance Analysis and Detailed Review Paper required for Organisation for Economic Co-operation and Development Test Guideline acceptance. We anticipate, however, that it also will be useful for accessing and comparing Pig-a data to data from other assays and for conducting quantitative assessments of Pig-a gene mutation responses. Environ. Mol. Mutagen., 60:759-762, 2019. © 2019 Wiley Periodicals, Inc.

中文翻译：

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Pig-a基因突变数据库。

X连锁的磷脂酰肌醇聚糖A类基因（Pig-a）中的突变导致糖基磷脂酰肌醇（GPI）锚和GPI锚定蛋白从红细胞和其他哺乳动物细胞的表面丢失。Pig-a基因突变检测通过免疫荧光标记和流式细胞术定量检测体内基因突变，以检测外周血红细胞上GPI锚定蛋白的丢失。作为对该测定法的监管接受的一部分，已创建了一个公共数据库，该数据库提供了有关在大鼠和小鼠中进行的Pig-a基因突变测定的详细信息。该数据库的可搜索版本可通过马里兰大学药学院设计和托管的网站获得。目前，该数据库仅包含鼠标和老鼠数据，但预计它将扩展到包括其他物种（包括人类）的数据。开发数据库的主要目的是协助编写回顾性的绩效分析和详细的审查文件，以供接受经济合作与发展组织测试指南。但是，我们预计，它对于访问和比较Pig-a数据与其他测定的数据以及对Pig-a基因突变反应进行定量评估也将很有用。环境。大声笑 Mutagen。，60：759-762，2019.©2019 Wiley Periodicals，Inc. 但是，我们预计，它对于访问和比较Pig-a数据与其他测定的数据以及对Pig-a基因突变反应进行定量评估也将很有用。环境。大声笑 Mutagen。，60：759-762，2019.©2019 Wiley Periodicals，Inc. 但是，我们预计，它对于访问和比较Pig-a数据与其他分析的数据以及对Pig-a基因突变反应进行定量评估也将很有用。环境。大声笑 Mutagen。，60：759-762，2019.©2019 Wiley Periodicals，Inc.