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In vitro effect of the crude extract of a potato common scab streptomycete in Sinaloa, Mexico.
Revista Argentina de Microbiología ( IF 1.9 ) Pub Date : 2019-02-22 , DOI: 10.1016/j.ram.2018.09.002
Amanda Alejo 1 , Eleuterio Burgueño 2 , Luis A Maldonado 3 , Gabriel Herrera 4 , Ruben Felix 5 , Erika T Quintana 1
Affiliation  

A strain isolated from potato common scab superficial lesions in El Fuerte Valley in northern Sinaloa, Mexico, was identified by 16S rRNA and morphological methods. Moreover, the effects of the crude extract of strain V2 was evaluated on radish and potato. The isolate was similar to Streptomyces acidiscabies in its morphological properties; however, the 16S rRNA gene sequence of strain V2 was neither 100% identical to this species nor to the streptomycetes previously reported in Sinaloa, Mexico. Strain V2 did not amplify any specific PCR products for genes nec1 and tomA, which have been found and reported in S. acidiscabies. Strain V2 produced a PCR product for the txtAB operon, which is related to the production of thaxtomin. In vitro assays using crude thaxtomin extract and a spore suspension of the organism caused necrotic symptoms on radish and potato, which were highly virulent in potato. This study reports that Streptomyces sp. V2 has a toxigenic region (TR) that is associated with the thaxtomin gene cluster.



中文翻译:

墨西哥锡那罗亚州马铃薯常见sc星霉菌丝的粗提物的体外作用。

通过16S rRNA和形态学方法鉴定了从墨西哥北部锡那罗亚州El Fuerte谷地马铃薯常见sc疮表皮病变中分离出的菌株。此外,评价了菌株V2的粗提物对萝卜和马铃薯的作用。该分离物的形态学特性与酸链霉菌相似。但是,菌株V2的16S rRNA基因序列与该物种或先前在墨西哥的锡那罗亚州报道的链霉菌都没有100%相同。应变V2的基因没有扩增任何特异性PCR产物nec1托马,已发现并报告的小号嗜酸菌。菌株V2为txtAB生产了PCR产物操纵子,与thaxtomin的生产有关。使用粗毒素的提取物和该生物的孢子悬浮液进行的体外分析导致萝卜和马铃薯的坏死症状,而马铃薯和马铃薯中的毒力很强。这项研究报告链霉菌属。V2具有与thaxtomin基因簇相关的产毒区(TR)。

更新日期:2019-02-22
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