Natural oils are traditional medicinal herbs, which have attracted interests for its potential anti-inflammatory and anticancer activities. The present work is aimed at evaluating the protective effect of garlic oil and cinnamon oil on diethylnitrosamine- (DENA-) and 2-acetylaminofluorene- (2-AAF-) induced p53 gene mutation and hepatocarcinogenesis in rats. Forty male albino rats were divided into 4 equal groups: control, hepatocellular carcinoma (HCC), garlic oil-HCC, and cinnamon oil-HCC. The HCC-induced group showed a significant decrease in the body mass and a significant elevation in the liver weight, alpha-fetoprotein (AFP), liver enzymes, hepatic malondialdehyde (MDA), and p53 protein expression levels as well as genetic mutations in intron 5 of p53 gene in the form of Single-Nucleotide Polymorphisms (SNPs) and insertions. In addition, the glutathione (GSH) level and superoxide dismutase (SOD) activities were increased. While HCC rats pretreated with garlic oil or cinnamon oil were significantly reversed, these destructive actions increased GSH and SOD levels. The HCC-induced group showed histopathological features of liver cancer including hypercellularity, nuclear hyperchromasia, mitotic figures, and preneoplastic foci. On the other hand, HCC rats pretreated with garlic oil or cinnamon oil revealed partial reversal of normal liver architecture. The present findings proposed that these natural oils have the ability to improve liver function, significantly reduced the liver toxicity and HCC development. However, further sophisticated studies are recommended before their use as conventional therapeutics for HCC treatment.

中文翻译：

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大蒜和肉桂油对白化大鼠肝细胞癌的保护作用。

天然油是传统的草药，因其潜在的抗炎和抗癌活性而引起了人们的关注。本工作旨在评估大蒜油和肉桂油对大鼠二乙基亚硝胺-（DENA-）和2-乙酰氨基芴-（2-AAF-）诱导的p53基因突变和肝癌发生的保护作用。将40只雄性白化病大鼠分为4组：对照组，肝细胞癌（HCC），大蒜油-HCC和肉桂油-HCC。HCC诱导组的体重显着下降，肝脏重量，甲胎蛋白（AFP），肝酶，肝丙二醛（MDA）和p53蛋白表达水平以及内含子的遗传突变显着增加单核苷酸多态性（SNP）和插入形式的p53基因的5位。此外，谷胱甘肽（GSH）水平和超氧化物歧化酶（SOD）活性增加。尽管用大蒜油或肉桂油预处理的HCC大鼠明显逆转，但这些破坏性作用增加了GSH和SOD水平。HCC诱导的组显示了肝癌的组织病理学特征，包括细胞过度增生，核增生，核分裂象和肿瘤前病灶。另一方面，用大蒜油或肉桂油预处理的HCC大鼠显示出正常肝脏结构的部分逆转。本研究结果表明，这些天然油具有改善肝功能，显着降低肝毒性和肝癌发展的能力。但是，建议在将其用作HCC治疗的常规疗法之前进行更深入的研究。