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Rapid and Sensitive Determination of Methylxanthines in Commercial Brands of Tea Using Ultra-High-Performance Liquid Chromatography-Mass Spectrometry.
International Journal of Analytical Chemistry ( IF 1.8 ) Pub Date : 2019-11-03 , DOI: 10.1155/2019/2926580 Ahmad Aqel 1 , Ahmed Almulla 1 , Asma'a Al-Rifai 2 , Saikh M Wabaidur 1 , Zeid A ALOthman 1 , Ahmed-Yacine Badjah-Hadj-Ahmed 1
International Journal of Analytical Chemistry ( IF 1.8 ) Pub Date : 2019-11-03 , DOI: 10.1155/2019/2926580 Ahmad Aqel 1 , Ahmed Almulla 1 , Asma'a Al-Rifai 2 , Saikh M Wabaidur 1 , Zeid A ALOthman 1 , Ahmed-Yacine Badjah-Hadj-Ahmed 1
Affiliation
Recently, chromatographic techniques have the potential to be greener in order to reduce the environmental impact. In this work, a new simple, sensitive, efficient, and green analytical method based on UHPLC-MS has been developed for a quick determination of methylxanthines including caffeine, theobromine, and theophylline in tea. Under the optimum conditions, a baseline separation has been achieved within 30 seconds, using isocratic elution consisting of 90% water and only 10% acetonitrile at 0.5 mL/min flow rate (3 mL acetonitrile per hour). The mass spectrometer was operated with the SIR mode in ESI+. The developed method was found to be linear in the range of 0.03–5 μg/mL, with correlation coefficients greater than 0.9995 for the three compounds. The respective values of LOD were found to be 0.025, 0.015, and 0.01 μg/mL for caffeine, theobromine, and theophylline, respectively. The proposed assay was applied to 30 commercial tea samples of different brands. Both caffeine and theobromine were found in all tea samples with maximum concentration in sample no. 15, corresponding to 32.6 and 2.72 mg/g of caffeine and theobromine, respectively. On the contrary, theophylline was not detected at all in most samples. When compared with all previous studies that dealt with the same compounds in different matrices, the developed method was found to be the fastest, allowing high-throughput analyses with more than 100 samples/h. The results prove that the method is suitable for routine analysis of methylxanthines and to distinguish the quality of tea samples of various brands.
中文翻译:
使用超高效液相色谱-质谱法快速灵敏地测定商品茶叶中的甲基黄嘌呤。
最近,色谱技术可能会更环保,以减少对环境的影响。在这项工作中,开发了一种基于UHPLC-MS的新的简单,灵敏,高效和绿色的分析方法,用于快速测定茶叶中的甲基黄嘌呤,包括咖啡因,可可碱和茶碱。在最佳条件下,使用等度洗脱由90%水和仅10%乙腈组成,流速为0.5 mL / min(每小时3 mL乙腈),可在30秒内实现基线分离。质谱仪在ESI +中以SIR模式运行。所提出的方法被发现是在0.03-5的范围内的线性 μg / mL,三种化合物的相关系数均大于0.9995。发现LOD的各自值为0.025、0.015和 0.01μ咖啡因,可可碱和茶碱的含量分别为g / mL。拟议的分析方法适用于30个不同品牌的商品茶样品。在所有茶叶样品中都发现了咖啡因和可可碱,最大样品中的咖啡因浓度最高。15分别相当于32.6和2.72 mg / g咖啡因和可可碱。相反,在大多数样品中根本没有检测到茶碱。与以前在不同基质中处理相同化合物的所有研究相比,该开发的方法被发现是最快的,可以进行每小时100多个样品的高通量分析。结果证明该方法适用于甲基黄嘌呤的常规分析,并能区分各种品牌茶样品的质量。
更新日期:2019-11-03
中文翻译:
使用超高效液相色谱-质谱法快速灵敏地测定商品茶叶中的甲基黄嘌呤。
最近,色谱技术可能会更环保,以减少对环境的影响。在这项工作中,开发了一种基于UHPLC-MS的新的简单,灵敏,高效和绿色的分析方法,用于快速测定茶叶中的甲基黄嘌呤,包括咖啡因,可可碱和茶碱。在最佳条件下,使用等度洗脱由90%水和仅10%乙腈组成,流速为0.5 mL / min(每小时3 mL乙腈),可在30秒内实现基线分离。质谱仪在ESI +中以SIR模式运行。所提出的方法被发现是在0.03-5的范围内的线性 μg / mL,三种化合物的相关系数均大于0.9995。发现LOD的各自值为0.025、0.015和 0.01μ咖啡因,可可碱和茶碱的含量分别为g / mL。拟议的分析方法适用于30个不同品牌的商品茶样品。在所有茶叶样品中都发现了咖啡因和可可碱,最大样品中的咖啡因浓度最高。15分别相当于32.6和2.72 mg / g咖啡因和可可碱。相反,在大多数样品中根本没有检测到茶碱。与以前在不同基质中处理相同化合物的所有研究相比,该开发的方法被发现是最快的,可以进行每小时100多个样品的高通量分析。结果证明该方法适用于甲基黄嘌呤的常规分析,并能区分各种品牌茶样品的质量。