当前位置: X-MOL 学术Connect. Tissue Res. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
The effects of age on the severity of joint damage and intra-articular inflammation following a simulated medial meniscus injury in 3, 6, and 9 month old male rats.
Connective Tissue Research ( IF 2.9 ) Pub Date : 2019-08-23 , DOI: 10.1080/03008207.2019.1641495
Kyle D Allen 1 , Kiara M Chan 1 , Elena G Yarmola 1 , Yash Y Shah 2 , Brittany D Partain 1
Affiliation  

Purpose: Aging is a known risk factor for osteoarthritis (OA). Several transgenic rodent models have been used to investigate the effects of accelerated or delayed aging in articular joints. However, age-effects on the progression of post-traumatic OA are less frequently evaluated. The objective of this study is to evaluate how animal age affects the severity of intra-articular inflammation and joint damage in the rat medial collateral ligament plus medial meniscus transection (MCLT+MMT) model of knee OA.Methods: Forty-eight, male Lewis rats were aged to 3, 6, or 9 months old. At each age, eight rats received either an MCLT+MMT surgery or a skin-incision. At 2 months post-surgery, intra-articular evidence of CTXII, IL1β, IL6, TNFα, and IFNγ was evaluated using a multiplex magnetic capture technique, and histological evidence of OA was assessed via a quantitative histological scoring technique.Results: Elevated levels of CTXII and IL6 were found in MCLT+MMT knees relative to skin-incision and contralateral controls; however, animal age did not affect the severity of joint inflammation. Conversely, histological investigation of cartilage damage showed larger cartilage lesion areas, greater width of affected cartilage, and more evidence of hypertrophic cartilage damage in MCLT+MMT knees with age.Conclusions: These data indicate the severity of cartilage damage subsequent to MCLT+MMT surgery is related to the rat's age at the time of injury. However, despite greater levels of cartilage damage, the level of intra-articular inflammation was not necessarily affected in 3, 6, and 9 month old male rats.

中文翻译:

年龄对 3、6 和 9 个月大雄性大鼠模拟内侧半月板损伤后关节损伤和关节内炎症严重程度的影响。

目的:衰老是骨关节炎 (OA) 的已知危险因素。几种转基因啮齿动物模型已被用于研究关节关节加速或延迟衰老的影响。然而,年龄对创伤后 OA 进展的影响较少被评估。本研究的目的是评估动物年龄如何影响膝关节 OA 大鼠内侧副韧带加内侧半月板横断 (MCLT+MMT) 模型中关节内炎症和关节损伤的严重程度。 方法:48,男性 Lewis大鼠年龄为 3、6 或 9 个月大。在每个年龄,八只大鼠接受了 MCLT + MMT 手术或皮肤切口。术后 2 个月,使用多重磁捕获技术评估 CTXII、IL1β、IL6、TNFα 和 IFNγ 的关节内证据,通过定量组织学评分技术评估OA的组织学证据。结果:与皮肤切口和对侧对照相比,MCLT+MMT膝盖中发现CTXII和IL6水平升高;然而,动物年龄并不影响关节炎症的严重程度。相反,软骨损伤的组织学调查显示,随着年龄的增长,MCLT+MMT 膝关节软骨损伤面积更大,受累软骨的宽度更大,并且有更多肥厚性软骨损伤的证据。与受伤时大鼠的年龄有关。然而,尽管软骨损伤程度更高,但 3、6 和 9 个月大的雄性大鼠的关节内炎症水平不一定受到影响。
更新日期:2019-11-01
down
wechat
bug