By targeted deletion of either the FUT1- or FUT2-gene for α1,2-fucosyltransferase, expression of FGM1 and FGA1, in murine testis was revealed to be sustained through unique interchangeability of the genes, indicating their significant roles for spermatogenesis. Accordingly, we examined the amounts of FGM1 and FGA1 in the testes of mice at 1–42 days after birth in comparison to those of several glycolipids including seminolipid. Although Forssman antigen and GM1 were present in relatively constant amounts during the period examined, GM3, which was the major one at 1 day, quickly decreased during development and had completely disappeared at 4 weeks. The following glycolipids were expressed in stage-specific manners, FGM1 for primary spermatocytes at 1 week, a seminolipid for secondary spermatocytes at 2 weeks, and GM3 lactone and FGA1 for spermatids and spermatozoa at 3 weeks. In fact, immunohistochemical staining with anti-FGM1 and anti-FGA1 antibodies demonstrated that FGM1 and FGA1 were distributed in the spermatocytes, and the spermatids and spermatozoa, respectively, and FGA1, together with seminolipid, were the immunogenic markers of spermatozoa. Thus, the fucosylation of glycolipids is a spermatogenesis-associated event, which should occur even with use of either the FUT1- or FUT2-gene.

中文翻译：

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精子发生相关的岩藻糖基化糖脂在小鼠睾丸中的变化。

通过任一的定向缺失FUT1 -或FUT2鼠睾丸中α1,2-岩藻糖基转移酶的基因，FGM1和FGA1的表达通过基因的独特互换性得以维持，表明它们在精子发生中的重要作用。因此，与几种糖脂（包括半脂质）相比，我们在出生后1–42天检查了小鼠睾丸中FGM1和FGA1的含量。尽管在检查期间Forssman抗原和GM1以相对恒定的量存在，但GM3（在第1天成为主要抗原）在发育过程中迅速下降，并在4周时完全消失。以下糖脂以阶段特定的方式表达，FGM1在第1周表达为原代精子细胞，半脂类在2周表达为次生精子细胞，GM3内酯和FGA1在3周表达为精子和精子。事实上，用抗FGM1和抗FGA1抗体进行的免疫组织化学染色显示，FGM1和FGA1分布在精子细胞中，精子和精子分别与FGA1以及半脂质一起成为精子的免疫原性标记。因此，糖脂的岩藻糖基化是与精子发生相关的事件，即使使用任何一种FUT1 -或FUT2 -基因。