Heterologous production of fungal ligninolytic cocktails is challenging due to the low yields of catalytically active lignin modifying peroxidases. Production using a natural system, such as a wood-rotting fungus, is a promising alternative if specific or preferential induction of the ligninolytic activities could be achieved. Using transcriptomics, gene expression of the white-rot Dichomitus squalens during growth on mixtures of aromatic compounds, with ring structures representing the two major lignin sub-units, was compared to a wood substrate. Most of the genes encoding lignin modifying enzymes (laccases and peroxidases) categorised as highly or moderately expressed on wood were expressed similarly on aromatic compounds. Higher expression levels of a subset of manganese and versatile peroxidases was observed on di- compared to mono-methoxylated aromatics. The expression of polysaccharide degrading enzymes was lower on aromatic compounds compared to wood, demonstrating that the induction of lignin modifying enzymes became more specific. This study suggests potential for aromatic waste streams, e.g. from lignocellulose pretreatment, to produce a lignin-specific enzyme cocktail from D. squalens or other white-rot fungi.

中文翻译：

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芳香族化合物的混合物诱导木质腐烂真菌Dhimitus squalens中木质素分解基因的表达。

由于催化活性的木质素修饰过氧化物酶的收率低，真菌木质素分解鸡尾酒的异源生产具有挑战性。如果可以实现木质素分解活性的特异性或优先诱导，则使用天然系统（例如木腐真菌）进行生产是一种有前途的选择。使用转录组学，将白腐烂的铁鞭毛藻在芳香化合物混合物上的生长过程中的基因表达与木质基质进行了比较，这些芳香化合物的环状结构代表了两个主要的木质素亚基。分类为在木材上高度或中度表达的大多数编码木质素修饰酶（漆酶和过氧化物酶）的基因在芳族化合物上的表达也相似。与单甲氧基化的芳族化合物相比，di-上观察到了一部分锰和通用过氧化物酶的较高表达水平。与木材相比，在芳香族化合物上多糖降解酶的表达要低，这表明木质素修饰酶的诱导变得更加特异性。这项研究表明，芳香废料流（例如来自木质纤维素预处理的废物）有可能从角鲨（D. squalens）或其他白腐真菌中产生木质素特异性酶混合物。