Following the recommended use of the inactivated poliovirus vaccine from Sabin strains (sIPV) by the WHO, a D antigen-specific neutralizing monoclonal antibody (mAb) 1G10 that recognized the human poliovirus type 1 Sabin strain (PV-I Sabin) was produced for D-antigen potency evaluation on sIPV. Study of the mAb 1G10 showed that it recognized a discontinuous conformational epitope of PV-I Sabin antigen. To identify this epitope quickly, easily and cost-effectively, clues to the epitope's identity were first obtained by employing a novel mimotope strategy based on a phage display library and "in silico" prediction. Then, the conformation of the epitope region, including the amino acid sequence, neutralizing sites, and location of this epitope, was identified using several classic epitope-mapping methods such as synthesized peptides analysis, neutralization assay and site-directed mutagenesis. The mimotope strategy may offer some guidance for achieving epitope identification in a more feasible and effective way. This mAb could be used in an in-house or national and international standard IPV D-antigen potency ELISA kit in the future.

中文翻译：

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使用模拟表位策略鉴定单克隆抗体1G10识别的人1型脊髓灰质炎病毒Sabin株中的表位。

在WHO推荐使用Sabin株（sIPV）的灭活脊髓灰质炎疫苗后，生产了一种D抗原特异性中和单克隆抗体（mAb）1G10，该抗体识别人1型脊髓灰质炎病毒Sabin株（PV-1 Sabin）。 -sIPV的抗原效力评估。对mAb 1G10的研究表明，它可以识别PV-1 Sabin抗原的不连续构象表位。为了快速，轻松和经济高效地鉴定该表位，首先通过采用基于噬菌体展示库和“计算机模拟”预测的新型模拟表位策略来获得表位身份的线索。然后，表位区域的构象，包括氨基酸序列，中和位点和该表位的位置，使用几种经典的表位作图方法，如合成的肽分析，中和测定和定点诱变，可以鉴定出这种蛋白。模拟表位策略可以为以更可行和有效的方式实现表位鉴定提供一些指导。该mAb将来可用于内部或国家和国际标准IPV D抗原效能ELISA试剂盒。