Direct analysis and identification of biological tissue is significant for clinical applications. In this study, porcine liver and kidney have been analyzed using laser ablation electrospray ionization time-of-flight mass spectrometry (LAESI-TOFMS). This method showed good reproducibility for the same types of tissue and is capable of distinguishing different tissue species. The margin assessment was also performed using porcine renal tissue, and the response time was less than 6 s. Furthermore, human hepatocarcinoma tissue and normal tissue were identified using this method. Our results indicate that LAESI-TOFMS is a feasible approach for direct identification of tumor tissue and potential for assessment of the resection margin.

中文翻译：

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用于直接分析生物组织的激光烧蚀电喷雾电离飞行时间质谱。

生物组织的直接分析和鉴定对于临床应用具有重要意义。在这项研究中，已使用激光消融电喷雾电离飞行时间质谱（LAESI-TOFMS）分析了猪肝和肾。此方法对相同类型的组织显示出良好的可重复性，并且能够区分不同的组织种类。还使用猪肾组织进行了切缘评估，并且反应时间少于6 s。此外，使用该方法鉴定了人肝癌组织和正常组织。我们的结果表明，LAESI-TOFMS是直接鉴定肿瘤组织和评估切除切缘潜力的可行方法。