Abstract

Four dimeric disintegrins were isolated from the venom of the steppe viper V. ursinii using liquid chromatography. Disintegrins prevented adhesion of MCF7 cells to fibronectin, which indicates their interaction with integrin receptors of the αVβ1 type. According to mass spectrometry data, the molar masses of disintegrins are about 14 kDa. The method of peptide mapping established the structure of a new heterodimeric disintegrin weighing 13 995.5 Da and shows that it belongs to the class of RGD/KGD-containing disintegrins.

中文翻译：

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草原Vi蛇V. ursinii毒液的二聚解毒蛋白。

摘要

使用液相色谱法从草原蛇蝎V. ursinii的毒液中分离出四个二聚解整合素。整联蛋白阻止了MCF7细胞与纤连蛋白的粘附，这表明它们与αVβ1型整联蛋白受体的相互作用。根据质谱数据，整联蛋白的摩尔质量为约14kDa。肽图分析方法建立了一个新的异二聚体整联蛋白的结构，重达13 995.5 Da，表明它属于含RGD / KGD的整联蛋白。