This study evaluated the structural and performance differences between arabinoglucuronoxylan micro-hydrogels that were enzymatically produced from alkaline-extracted wheat bran arabinoglucuronoxylans using recombinant α-L-arabinofuranosidase (AbfB) that selectively removes arabinose side chains, and chemically through coacervation process, as delivery devices for bioactive substances. The encapsulations of model bioactive substance, gallic acid (GA), in the hydrogels, were done either in-situ or ex-situ to identify the most effective encapsulation and delivery method. The hydrogels particle size distribution, polydispersity index, GA encapsulation efficiency, retention and release of functional GA (based on antioxidant activity) were assessed. The hydrogels formed in both coacervation and enzymatic processes had particle size ranges of 469–678 nm, which classify them as micro-hydrogels. However, the latter were monodispersed with polydispersity index (PdI) < 0.4 compared to the former with PdI > 0.7. In addition, enzymatically produced hydrogels attained higher zeta potential (−8.8 mV) and retained and released GA with higher anti-oxidant capacity (91%) than chemically formed micro-hydrogels (zeta potential = − 3.3 mV and antioxidant capacity = 80%). However, GA encapsulation efficiencies (72% in-situ and 68% ex-situ) were higher in chemically formed micro-hydrogels than enzymatically produced micro-hydrogels (59% in-situ and 52% ex-situ). The in-situ encapsulated GA experienced less initial burst during sustained release of 8 h compared to ex-situ encapsulation. Overall, enzymatic modification process and in-situ encapsulation were the most effective methods for production of arabinoglucuronoxylan micro-hydrogels delivery devices and for encapsulation of the GA, respectively, because of maintaining functional GA upon release and having the potential to customize the structural and functional properties of the micro-hydrogels.

中文翻译：

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麦麸阿拉伯木聚糖制成的水凝胶的性能和结构比较，使用酶促和凝聚法作为微囊和纳米囊封和递送装置。

这项研究评估了使用碱性α-L-阿拉伯呋喃糖苷酶（AbfB）选择性地去除阿拉伯糖侧链并通过凝聚过程化学方法作为输送装置从碱性提取的小麦麸皮阿拉伯葡糖醛酸木聚糖酶促生产的阿拉伯葡糖醛酸木聚糖微水凝胶的结构和性能差异。用于生物活性物质。模型生物活性物质没食子酸（GA）在水凝胶中的包裹是原位或异位完成的，以确定最有效的包裹和递送方法。评估了水凝胶的粒度分布，多分散指数，GA包封效率，功能性GA的保留和释放（基于抗氧化剂活性）。在凝聚和酶促过程中形成的水​​凝胶的粒径范围为469–678 nm，将它们归类为微水凝胶。但是，后者是单分散的，多分散指数（PdI）<0.4，而前者是PdI> 0.7。此外，与化学形成的微水凝胶（ζ电位=-3.3 mV，抗氧化能力= 80％）相比，酶促生产的水凝胶具有更高的zeta电位（-8.8 mV），并且保留和释放的GA具有更高的抗氧化能力（91％）。 。但是，化学方法制备的微水凝胶的GA封装效率（原位为72％，原位为68％）要比酶促生产的微水凝胶（原位为59％，原位为52％）要高。与异位包封相比，原位包封的GA在持续释放8 h期间经历了较少的初始爆发。总体，